摘要
目的:建立肿瘤神经新生现象的体外研究模型,探讨在体外共培养模式下,人舌鳞状细胞癌细胞系HN-12对Sprague-Dawley(SD)大鼠三叉神经节(TG)突起生长的影响。方法:无菌条件下将HN-12细胞与SD大鼠TG接种于6孔板的Matrigel胶内进行共培养,TG单独培养作为对照组。分别于培养的第24、48、72、96、120 h拍照,测量实验组和对照组TG节神经突起生成的长度,利用SPSS19.0软件包检测2组神经突长度有无显著差异。结果:连续培养5 d发现,在共培养组,HN-12细胞对神经节突起的形成和生长具有明显的促进作用,与对照组相比有显著差异。并且,实验组中神经节突起具有明显的趋肿瘤细胞生长特性。结论:舌鳞癌神经新生现象体外培养模型成功建立。HN-12细胞具有促神经节突起生长的作用。
PURPOSE： To build a new in vitro study model of neoneurogenesis, to study the influence of HN12 cell on trigeminal ganglion of SD rat under the condition of coculture. METHODS： Mouse trigeminal ganglion（TG） and human oral squamous cancer cells（HN12） were co-cultured in Matrigel matrix. Control cultures consisted of oral squamous cancer cells alone and TG alone. Neurite outgrowth was quantitated with dark phase microscopy and image analysis（Optimas 6.1） at 24,48,72,96 and 120 h. SPSS 19.0 software package was used for statistical analysis. RESULTS： HN-12 cell had a strong influence on the neurites growth compared with control group after 5 days of consecutive observation.CONCLUSION： HN-12/TG coculture model was successfully built. HN-12 cell has a strong influence on the neurites growth.
出处
《中国口腔颌面外科杂志》
CAS
2014年第4期307-310,共4页
China Journal of Oral and Maxillofacial Surgery
基金
上海交通大学医工交叉研究基金(YG2011MS58)~~
