摘要
目的观察并探讨在体外坐骨神经与腺样囊性癌细胞共培养中腺样囊性癌细胞对由坐骨神经长出的雪旺氏细胞生长、迁移的影响。方法采用细胞培养技术将腺样囊性癌细胞、舌癌细胞及成纤维细胞分别与小鼠坐骨神经共培养;制备腺样囊性癌细胞与坐骨神经共培养细胞玻片,并行S100免疫组化染色实验。结果坐骨神经与腺样囊性癌细胞共培养中由坐骨神经长出的雪旺氏细胞向腺样囊性癌细胞群落迁移,表现出趋化性;舌癌细胞与成纤维细胞对雪旺氏细胞无诱向作用;经S100免疫组化染色,坐骨神经生长出长梭形的细胞,染色阳性,为雪旺细胞;多边形的细胞染色阴性,为成纤维细胞。结论坐骨神经与腺样囊性癌细胞共培养中长出的雪旺氏细胞向腺样囊性癌细胞群落趋化性生长,可能是腺样囊性癌嗜神经性重要机理之一,与腺样囊性癌细胞发生雪旺细胞分化有关。
Objective：To observe and study the effect of the salivary adenoid cystic carcinoma cell on the growth and migration of Schwarm cell deriving from the sciatic nerve in vitro co -culture. Methods：Co - culture of sciatic nerve with salivary adenoid cystic carcinoma cell , tongue cancer celland fibroblast respectively . The cells cultured from sciatic nerve was examined by immunohistochemistry technique. Results：In contrast to control group , the induction effect of the salivary adenoid cystic carcinoma cell on the Schwann cell was seen . Stained by immunocytochemistry with anti- S- 100protein,the Schwann＇ s cells were stained brown, but fibroblasts stainless. Condusion： The salivary adenoid cystic carcinoma cell could induce the growth of the Schwann cell. It may be one of the mechanism of the perineural invasion in salivary ACC , and this phenomena may have relationship with the Schwann cell dif- ferentiation in onco - myoepithelial cell of salivary ACC.
出处
《现代肿瘤医学》
CAS
2006年第5期518-520,共3页
Journal of Modern Oncology
基金
国家自然科学基金(30271424)
陕西省自然科学基金(2002C2-06)
关键词
腺样囊性癌
雪旺细胞
嗜神经侵袭
趋化性
免疫组织化学
adenoid cystic carcinoma
schwann cell
neural invasion
chemotactic growth
immunohistochemistry
co - culture
