摘要
为预测口蹄疫病毒（FMDV）VP1蛋白的重要功能残基,本研究利用进化踪迹及蛋白结构同源建模等方法对91株Asia 1型FMDV的VP1氨基酸序列进行分析,结果显示91个病毒株分属于B、C和D分支,2005年~2006年分离株处于另一个分支,命名为E分支。以10分区分析进化树,共34个组特异残基,其中140S、141S、146L、148A、149L、150A、151R、152R、153V、155N和156R分布于G-H环区域,预测它们与抗原性和受体结合特性有关;B、C、D和E 4个分支的组内特异残基分别为24S、153G、196H;59H、141P、146M、150S、169N;50I、135A和99N、127S、140S、151R,这些残基为各分支的分子标识;组特异残基35V、80L、93S、127A分布于VP1和其他结构蛋白的作用界面,可能对病毒的装配具有重要作用。本研究结果为FMDV的VP1功能研究提供了参考数据。
To predict important functional residues in VP1 protein of foot-and-mouth disease virus （FMDV）, VPI amino acid sequences of 91 serotype Asial strains were analyzed by using evolutionary trace and protein homology modeling methods. The results showed that most of the isolates belonged to the previous classification of B, C, and D branch, however, a new branch mainly composed with the isolates from 2005 to 2006 was named as E. While, thirty-four class specific trace residues were found by analyzing the tree with 10 partitions, and amino acid residues of 140S、141S、146L、148A、149L、150A、151R、152R、153V、155N and J56R were found in the G-H loop of VPI which were predicted to be the functional residues related with antigenicity and receptor-binding specificity. Evidently, the class specific residues of 24S, 153G and 196H might be served as molecular makers for branch B, 59H,141p, 146M, 150S and 169N for branch C, 50I, 135A for branch D and 9914, 127S, 140S, 151R for branch E, respectively. Class specific residues 35V, 80L, 938 and 127A were located at the interfaces between VP1 and other structural proteins and might play important roles in virus assembly. Those results provide references for the functional study of FMDV VP1 protein.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2014年第8期615-619,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
国家科技重大专项(2012XX10004214)
中国农业科学院基本科研业务费(0302012010)
关键词
Asia
1型口蹄疫病毒
VP1蛋白
踪迹进化分析
同源建模
Asia 1 serotype foot-and-mouth disease virus
VP1 protein
evolutionary trace analysis
homology modeling
