摘要
目的探讨中药白花蛇舌草提取物(HD-1)对人体癌细胞及小鼠实体瘤的抑制作用。方法对人体癌细胞采用MTT法进行体外实验,检测白花蛇舌草提取物HD-1对人肝癌细胞HepG2和人直结肠癌细胞株HCTI 16生长的抑制作用;对S180实体瘤小鼠模型进行体内实验,对比高、低剂量的白花蛇舌草提取物HD-1对荷瘤小鼠体质量、抑瘤率、胸腺指数、脾指数等指标的影响。结果体外实验显示,中药白花蛇舌草提取物对HepG2和HCTI16的抑制率和给药浓度、药物作用时间相关。体内实验显示,与模型对照组相比,高HD-1组、低HD-1组瘤质量均减轻,抑制率分别为13.28%,26.47%,脾脏指数明显升高(P<0.05)。CTX组与CTX+HD-1组比较,小鼠胸腺指数、脾脏指数、肝脏指数、肺脏指数均有显著性差异(P均<0.05)。结论中药白花蛇舌草提取物体外能够明显抑制人肝癌细胞HepG2和人直结肠癌细胞株HCTI 16的生长,能够明显抑制小鼠体内实体瘤的生长。
Objective It is to explore the inhibitory action of herb Hedyotis Diffusae （ TCM） extract HD-1 on human cancer cells and solid tumor in mice .Methods MTT assay was used in the vitro experiments on human cancer cells .Growth inhibition of herb Hedyotis Diffusae extract HD -1 was analyzed in HepG2 cells and human colorectal cancer cell lines HCTI 16.Results of weight of tumor -bearing mice, tumor inhibition rate, thymus index and spleen index were compared on S 180 solid tumor in vivo mouse model between high -dose group and low dose group .Results In vitro experiments , inhibition rate of HD-1 extract on HepG2 and HCTI 16 were related to drug concentrations and drug effect time .In vivo experiments , the high HD-1group and low -HD-1 group tumor weight were reduced compared with the model group .Inhibition rates were 13.28%, 26.47%respectively.Spleen index in the high HD -1 group and low-HD-1 group was significantly higher than that in the model group （P〈0.05）.In the CTX group and CTX＋HD-1 group, thymus index and spleen index , liver index, lung index were statistically significant （P〈0.05）.Concl usion Herba hedyotis diffusae （TCM） extract could inhibit HepG2 cells and human colon cancer cell lines HCTI 16 straight growth, and inhibit the growth of solid tumors in mice .
出处
《现代中西医结合杂志》
CAS
2014年第25期2764-2766,共3页
Modern Journal of Integrated Traditional Chinese and Western Medicine
关键词
白花蛇舌草提取物
癌细胞
小鼠实体瘤
herba Hedyotis Diffusae extract
cancer cell
mice solid tumor
