摘要
目的:建立黔产一枝黄花药材HPLC指纹图谱。方法:采用高效液相色谱法,以Diamonsil C18(200 mm×4.6 mm,5μm)为色谱柱,乙腈-0.2%磷酸溶液为流动相,梯度洗脱,检测波长为282 nm,流速1.0 mL·min-1,柱温25℃,分析了10批不同产地一枝黄花药材指纹图谱。结果:10批一枝黄花药材中有13个共有峰,经相似度计算,整体相似度良好。结论:该方法稳定、重复性好,可用于黔产一枝黄花药材的质量控制。
This study was aimed to establish the fingerprints of Qian Solidaginis Herba by HPLC. The fingerprints of Qian Solidaginis Herba were built by using Diamonsil C18 （200 mm × 4.6 mm, 5 μm） column and acetonitrile -0.2%H3PO4 aqueous solution in gradient as mobile phase. The flow rate was 1.0 mL·min-1. Detecting wavelength was set at 282 nm. The total 10 batches of Qian Solidaginis Herba samples and their adulterants were analyzed. The results showed that 13 peaks were identified as the fingerprints of Qian Solidaginis Herba. Under chromatographic conditions mentioned above. It was concluded that the HPLC fingerprints method was found to have satisfactory accuracy, sta-bility and reproducibility, which was a potential method for the quality control of Qian Solidaginis Herba.
出处
《世界科学技术-中医药现代化》
北大核心
2014年第7期1578-1582,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
贵州省科技厅中药现代化科技产业研究开发专项项目(黔科合中药字[2010]5028号):酢酱草
艾纳香
土知母等贵州民族药材指纹图谱及质量标准提高研究
负责人:冯华
贵州省中医药管理局中药
民族医药科学技术研究项目(QZYY2010-93):一枝黄花药材HPLC指纹图谱研究
负责人:冯华
