摘要
目的探讨DNA和结核抗体(TB-Ab)联合检测在结核性胸膜炎的临床诊断意义。方法分别采用斑点金免疫渗滤法和实时荧光定量PCR法对95例结核性胸腔积液患者及45例非结核性胸腔积液患者进行TB-Ab和结核分枝杆菌DNA检测,并对结果进行分析比较。结果结核性胸膜炎组结核分枝杆菌DNA检测阳性率为56.8%,显著高于非结核性胸膜炎组(4.4%),差异具有统计学意义(P<0.05)。结核性胸膜炎组TB-Ab检测阳性率为58.9%,显著高于非结核性胸膜炎组(6.6%),差异具有统计学意义(P<0.05)。两项指标应用结核性胸膜炎组阳性率可提高为78.9%,显著高于涂片镜检以及结核菌培养单项检测阳性率,差异具有统计学意(P<0.01)。结论结核分枝杆菌DNA与TB-Ab联合检测可明显提高结核性胸膜炎的阳性检出率,而且快速、简便、易操作。
Objective To disscuss the significance of combined detection of DNA and tuberculosis antibody（TB-Ab）in the diag-nosis of tuberculous pleurisy.Methods Dot immunogold filtration assay and real-time fluorescence PCR method were respectively used to detect TB-Ab and DNA of 95 cases of tuberculous pleural effusion patients（tuberculosis pleurisy group）and 45 cases of pa-tients without tuberculous pleural effusion（non-tuberculous pleurisy group）.Results were analyzed and compared.Results The positive rate of Mycobacterium tuberculosis DNA in tuberculosis pleurisy group was 5 6 .8%,which was higher than that of non-tu-berculous pleurisy group（4.4%）,with significant difference between them（P〈0.05）.The positive rate of TB-Ab in tuberculosis pleurisy group was 58.9%,higher than that of non tuberculous pleurisy group （6.6%）,and the difference was statistically signifi-cant （P〈0.05）.Combined application of DNA and TB-Ab,the positive rate of tuberculosis pleurisy group could be increased to 78.9%,significantly higher than the positive rates of individual test of smear or bacterial culture（P〈0.01）.Conclusion Combined detection of Mycobacterium tuberculosis DNA and TB-Ab can significantly improve the positive detection rate of tuberculous pleuri-sy,and the method is fast,simple and easy to operate.
出处
《国际检验医学杂志》
CAS
2014年第12期1612-1613,1615,共3页
International Journal of Laboratory Medicine
关键词
结核分枝杆菌
聚合酶链反应
抗酸染色
结核抗体
DNA
Mycobacterium tuberculosis
polymerase chain reaction
acid fast staining
DNA
tuberculosis antibody
