摘要
目的分析比较痰涂片、痰培养及FQ-PCR检查结核杆菌对肺结核的诊断价值。方法痰直接涂片和浓集涂片找抗酸杆菌;痰结核杆菌培养;FQPCR检测痰中TB-DNA。结果浓集法较直接法阳性率有大幅提高,培养阳性率较低,FQ-PCR法敏感度最高,三种方法检测结果具有很好的相关性。结论涂片检查结核杆菌应提倡浓集法,FQ-PCR法检测TB-DNA可列入TB实验诊断的常规项目,涂片、培养及FQPCR同时对结核杆菌进行检测可从不同方面满足临床的需要。
Objective To compare the diagnostic value of smearing, culturing and FQ-PCR on detecting TB for pulmonary tuberculosis.Methods TB was detected by smearing directly the concentrated sputum and by sputum culturing.TB-DNA in the sputum was detected by FQ-PCR.Results The positive rate of smearing the concentrated sputum was obviously higher than that of smearing directly.The positive rate of culturing methods was relatively low.The sensitivity of FQ-PCR was the highest.The result of detection by all three methods were highly interrelated.Conclusion Smearing concentrated sputum would be preferable instead of detecting TB directly in the sputum.Detecting TB-DNA by FQ-PCR might be adopted as a routine laboratory item for diagnosing pulmonary tuberculosis.It will meet clinical needs from different aspects to adopt smearing, culturing and FQ-PCR to detect TB.
出处
《同济大学学报（医学版）》
CAS
2005年第3期57-58,65,共3页
Journal of Tongji University(Medical Science)
