摘要
实验研究了重组Klebsiella pneumoniae批式发酵生产1,3-丙二醇过程中辅助碳源蔗糖与葡萄糖对发酵过程的影响,对发酵工艺进行了放大,并对流加策略进行了优化.结果表明,葡萄糖为发酵生产1,3-丙二醇的辅助碳源优于蔗糖;以重组Klebsiella pneumoniae为菌种,以葡萄糖为辅助碳源,采用指数流加策略,30 L发酵罐中1,3-丙二醇的产量最高达85.2 g/L,产率达0.63 mol/mol,比单纯以甘油为碳源分别提高37.35%和25.00%.
The effects of glucose and sucrose as two kinds of cosubstrate on fed-batch fermentation of 1,3-propanediol by recombinant Klebsiella pneumoniae were investigated, and the scale-up of fermentation technology and optimization of feeding strategy also studied. The results showed that glucose as cosubstrate was better than sucrose. The final yield and productivity of 1,3-propanediol were 85.2 g/L and 0.63 mol/mol in 30 L fermentor by exponential feeding strategy with glucose, respectively. Compared with only glycerol as the carbon source, the yield and productivity of 1,3- propanediol were raised by 37.35% and 25.00%, respectively.
出处
《过程工程学报》
CAS
CSCD
北大核心
2014年第4期660-663,共4页
The Chinese Journal of Process Engineering
基金
国家重点基础研究发展计划(973)资助项目(编号:2007CB714304)
国家高技术研究发展计划(863)资助项目(编号:2006AA020102
2006AA020103
2006AA020201)
