摘要
用粘质沙雷氏菌(Serratia marcescens)的菌悬液研究了该菌合成碱性蛋白酶的阻遏作用.八种碳源对产酶的影响各不相同,其中以琥珀酸及DL^(-1)苹果酸的阻遏作用最为显著.琥珀酸对产酶的调节随浓度增大,阻遏作用增强.且加入时间愈早,阻遏作用也愈强.其阻遏机制与利福平类似,与氯霉素则不同.cAMP能消除琥珀酸对产酶的阻遏作用,表明该阻遏作用属分解代谢物的阻遏.
Catabolite repression of Serratia marcescens producting alkaline protease was studied by using the cell suspensions. Eight carbon sources affected the production of protease differently. The repression of succinate and DL-malate was most effective among these. When the concentration of succinate in- creased, the repression of protease formation was enhanced. In addition, the repression of succinate on extracellular protease secretion was relative with time of adding it, namely, the earlier, the better. The repression mechanism of succinate was similar to that of rifamycin, but not same as that of chloram- phenicol. cAMP could overcome the reprecssion produced by succinate. The data suggested that exoen- zyme secretion was controlled by a mechanism of catablite repression.
出处
《武汉大学学报（自然科学版）》
CSCD
1993年第1期88-92,共5页
Journal of Wuhan University(Natural Science Edition)
关键词
碱性蛋白酶
粘质沙雷氏菌
阻遏作用
Alkaline protease
catabolite repression
Serratia marcescens
