摘要
为明确‘费乌瑞它’ב陇薯3号’杂种F1优良无性株系A1和A3及‘费乌瑞它’×J07-6杂种F1优良无性株系C2在染色体及DNA水平上的遗传差异,利用根尖染色体制片技术和SSR分子标记技术,对这3个优良株系的核型及SSR指纹特征进行分析。结果表明:(1)株系A1、A3和C2均为四倍体(2n=4x=48),其核型类型分别为2B、1B和1A;株系A1和A3核型公式均为2n=4x=48=36m+12sm,C2核型公式为2n=4x=48=48m。(2)利用筛选出的10对SSR适宜引物PCR扩增得到50个多态性位点,多态性位点百分率为79.37%。(3)建立了3个马铃薯杂种优良株系及其亲本的SSR指纹图,并以遗传距离(GD值)0.45为基准,将6个材料分为3类:母本‘费乌瑞它’、株系A1和父本‘陇薯3号’聚为一类,株系A3和C2聚为另一类,父本J07-6单独列为一类。
To identify the genetic differences among hybrid lines A1 and A3 derived from a cross of ‘Favorita’ × ‘Longshu 3’,and hybrid line C2 from a cross of ‘Favorita’ ×J07-6 in chromosome and DNA level,we analyzed their karyotype and SSR fingerprint characteristics using root tip chromosome technology and SSR molecular marker technology.The result showed that：（1）Hybrid line A1,A3 and C2 were tetraploid （2n 4x 48）,with karyotype type of 2B,1B and 1A,respectively.The karyotype formula of A1 and A3 were 2n 4x 48 36m＋12smand2n 4x 48 48m,respectively.（2）A total of 50 polymorphic loci were amplified using the 10 suitable SSR primer pairs,accounting for 79.37％ of all the amplified loci.（3）The SSR fingerprints of the 3 hybrid lines and their parents were established,which provided a basis for the identification of the hybrid lines and their parents at DNA level.The materials were classified into three categories based on genetic distance（GD） value of 0.45：mother favorite,hybrid line A1 and father ‘Longshu 3’ as a category,hybrid line A3 and C2 as a category,and father J07-6 as a category.
出处
《西北植物学报》
CAS
CSCD
北大核心
2014年第7期1318-1324,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家科技支撑计划(2012BAD02B05)
内蒙古自然科学基金重大项目(2013ZD03)
内蒙古马铃薯产业发展重大技术集成创新与示范项目(20131706)
国家现代农业产业技术体系建设专项资金资助(CARS-10-P17)
