摘要
目的:探讨针刺捻转补法和捻转泻法干预对自发性高血压大鼠(spontaneous hypertensive rats,SHR)心肌损害的效应差异。方法:将60只雄性11周SHR大鼠随机分为4组,即模型对照组(A)、捻转补法组(B)、捻转泻法组(C)、针刺留针组(D),每组15只,并以11周龄雄性Wistar大鼠12只作为空白对照组(E)。A组和E组不针刺,进行与其他组相同的抓捉捆绑刺激;B组针"太冲"穴,施捻转补法1min,留针9min;C组针"太冲"穴,施捻转泻法1min,留针9min;D组针"太冲"穴,不予以手法,留针10min,每天针刺1次,针刺6d休息1d,一共针刺24d。针刺前和针刺开始后每隔6天用无创血压仪测量大鼠收缩压和舒张压;28d后苏木精-伊红(HE)和三色(Masson)染色法观察左心室肥大、增生及心肌纤维化情况;酶联免疫法(Elisa)测定左心室心肌内皮素-1(ET-1)含量;PCR半定量法分析左心室肌Ⅰ、Ⅲ型胶原mRNA表达变化。结果:(1)血压:实验后A、B两组的收缩压和舒张压均有升高(P<0.05),C、D两组收缩压和舒张压均降低(P<0.05),C组的降压效应较D组更明显(P<0.05)。(2)HE和Masson染色显示:除E组外,A、B、C、D组均有心肌肥大和心肌纤维化,C组最轻,其次为D组,A组和B组均比较严重。(3)ET-1浓度:5组间ET-1浓度均有差异(均P<0.05),浓度由高到低依次为:A、B、D、C、E组。(4)Ⅰ型胶原mRNA:C组与D组的Ⅰ型胶原mRNA水平比较差异无统计学意义(P>0.05),C组与A、B组相比,水平较低(均P<0.05),E组的胶原水平最低;Ⅲ型胶原mRNA:A组与B组比较差异无统计学意义(P>0.05),C组与A、B、D组相比,C组的Ⅲ型胶原mRNA水平较低(P<0.05)。结论:捻转泻法可以降低SHR的收缩压和舒张压,能有效抑制ET-1的合成和Ⅰ、Ⅲ型胶原mRNA的表达,对Ⅲ型胶原mRNA抑制作用更明显,与捻转补法存在生物效应差异。
Objective To explore the effect differences between twirling-rotating reinforcing and reducing tech- nique of acupuncture on cardiac damage in spontaneous hypertensive rats （SHR）. Methods Sixty male ll-week- old SHR were randomly divided into four groups： a model control group （group A）, a twirling-rotating reinforcing technique group （group B）, a twirling-rotating reducing technique group （group C） and a needle retaining group （group D）, 15 rats in each one. In addition, twelve male ll-week-old Wistar rats were used as a blank control group （group E）. Acupuncture was not used in group A and group E, only with grasp, capture and binding stimulation that was also adapted in the rest groups. Rats in the group B were treated with acupuncture at ＂Taichong＂ （LR 3） by twirling^ro- rating reinforcing technique for 1 rain and then the needles were retained for 9 mira rats in the group C were treated with acupuncture at ＂Taichong＂ （LR 3） by twirling-rotating reducing technique for 1 min and then the needles were retained for 9 mira rats in the group D were treated with acupuncture at ＂Taichong＂ （LR 3） hut without any technique and then needles were retained for 10 min. Before and after acupuncture, blood pressure monitor was used to measure therats＇ systolic pressure and diastolic pressure every 6 days. Twenty-eight days after the treatment, HE and Masson staining were adopted to observe the status of left ventricular hypertrophy and myocardial fibrosis. ELISA method was applied to test the content of endothelin-1 （ET-1）. PCR semiquantitative method was used to analyze Type I and HI collagen mRNA in the left ventricular. Results （1） Blood pressure., after the treatment, the systolic pres- sure and diastolic pressure were both increased in the group A and the group B （P〈0.05） ; while the two pres- sures were both lowered in the group C and the group D （P〈0. 05）, which was more obvious in the group C （P〈0. 05）. （2） Aecording to HE and Masson staining, except for the group E, the myocardial hypertrophy and fibrosis could be found in the rest groups, in which the group C was the modest, followed by the group D, while the group A and the group B were more severe. （3） Concentration of ET-1 ： there were differences of concentration of ET-1 among 5 groups （P〈0.05）, and the concentration value from high to low was the group A, B, C, D and E. （4） Type I collagen mRNA., the difference of level of Type I collagen mRNA between group C and D was not statis- tically significant （P〉0.05） ; compared with the group A and B, the level was lower in the group C; the level was the lowest in the group E. Type III collagen mRNA： the difference between the group A and B was not statistically significant （P〉0.05）;compared with the group A, B and D, the level was lower in the group C. Conclusion The twir- ling-rotating reducing could reduce the systolic pressure and diastolic pressure in SHR, effectively prohibit the production of ET-1 and expression of Type I and Ⅲ collagen mRNA, and it has more obvious inhibiting effect on Type Ⅲcollagen mRNA. There is biological effect difference between twirling-rotating reinforcing and reducing technique.
出处
《中国针灸》
CAS
CSCD
北大核心
2014年第8期791-797,共7页
Chinese Acupuncture & Moxibustion
基金
国家自然科学基金项目:81072861
关键词
自发性高血压
捻转补法
捻转泻法
心肌损害
内皮素-1(ET-1)
I
Ⅲ型胶原mRNA
spontaneous hypertension~ twirlingarotating reinforcing technique
twirlingarotating reducing tech-nique
cardiac damagel endothelin-1 （ET-1）~ type I and I]I collagen mRNA
