摘要
目的:探讨在自发性高血压大鼠左室重构过程中肝细胞生长因子(HGF)的变化与心肌纤维化的关系。方法:①自发性高血压大鼠(SHR)18只,分别为6周龄,14周龄和24周龄,以18只WKY鼠为正常对照组,年龄和体质量配比,每组各6只,雌雄配对。②采用大鼠血压心率测定仪测量大鼠安静、清醒状态尾动脉收缩压。测量左心室质量。采用ELISA法测量血浆及心肌组织HGF浓度。心肌胶原容积(CVF)和血管周围胶原容积(PVCA)采用病理图像分析检测法。结果:各年龄组SHR血压水平、左室质量指数、血浆HGF浓度均高于对照组,心肌组织HGF浓度在14,24周龄时低于对照组。同对照组相比,CVF在14、24周龄之间有统计学差异(P<0.05)。在6周龄之间无统计学差异(P>0.05)。各年龄段PVCA均高于对照组。CVF,PVCA与血浆HGF浓度有显著正相关性(r=0.7284,P<0.01;r=0.7191,P<0.01〉,与组织HGF浓度呈负相关(r=-0.4245,P<0.05;r=-0.4749,P<0.05〉。结论:在自发性高血压大鼠左室重构过程中,组织HGF水平降低与心肌纤维化的形成密切相关,这可能与其抗纤维化作用减弱有关。
AIM：To evaluate the relationship between hepatocyte growth factor（ HGF ）and cardiac fibrosis during the left ventricular remodeling in SHR. METHODS： The blood samples and the heart speciments from 6,14,24 weeks old SHRs and age-matched Wistar-kyoto rats（ WKY） were studied. Each group is six. Systolic blood pressure was measured by the tail-cuff method. The plasma and myocardium HGF were determined by ELISA. The collagen volume fraction （ CVF ） and perivascular circumferential area （PVCA） were measured by pathological examination. RESULTS： ① The systolic blood pressure, left ventricular weight index and plasma HGF concentration were higher significantly in SHRs of three agegroups than WKY rats in age matched groups, but myocardium HGF concentration was significantly decreased in SHRs as compared with WKYs at 14 and 24 weeks of age, when the hearts showed hypertrophic changes induced by hypertension. ② Compared with WKYs in age - matched groups, CVF and PVCA were increased significantly in SHRs of three age - groups, but significantly differences of CVF was found at 14 and 24 weeks; and PVCA showed differences at each stage. ③ Tissue HGF showed significantly correlation with PVCA and CVF（ r = - 0. 4245 ,P 〈 0.05 ; r = - 0. 4749, P 〈 0.05 ）. CONCLUSION ： The lowing tissue HGF is related to cardiac fibrosis in the development of cardiac remodeling due to its antifibrotic action reducing.
出处
《心脏杂志》
CAS
2005年第4期323-325,329,共4页
Chinese Heart Journal
关键词
肝细胞生长因子
左室重构
心肌纤维化
SHR
hepatocyte growth factor
left ventricular remodeling
cardiac fibrosis
SHR
