摘要
了解2011年石家庄地区流感样病例（Influenza-like Illness,ILI）病毒感染及流行情况,为呼吸道感染的诊断和防治提供科学依据。在监测点医院采集ILI咽拭子标本483例,采用多重RT-PCR检测15种呼吸道病毒。483例ILI中病毒阳性的有214例,总阳性率为44.31%,依次为腺病毒（ADV）和肠道病毒（HEV）各8.70%、呼吸道合胞病毒（RSV）A 8.07%、鼻病毒（HRV）7.45%、甲型流感病毒（FLuA）5.38%、冠状病毒（HCoV）OC43/HKU1型和副流感病毒（PIV）3型各2.90%、偏肺病毒（HMPV）1.86%、PIV1型1.24%、HCoV229E/NL63型和PIV2型各1.04%、博卡病毒（HBoV）0.83%和乙型流感病毒（FLuB）0.41%。两种及以上病毒混合感染26例（5.38%）,以HEV/HRV合并其他病毒检出占首位。全年均可检出病毒感染病例,检出高峰在1~2月。ADV和HRV几乎全年均可检出,无明显季节性,HEV在4~11月均可检出,流行高峰在夏秋季,FLuA/B流行主要集中在冬春季,RSV检出全部为A亚型,PIV检出以3型为主,HCoV-OC43/NL63型阳性率明显高于229E/NL63型。2011年石家庄地区ILI中前五位病毒为HEV、ADV、RSV-A、HRV和FluA,各病毒随时间不同而各具其流行特征。
This study aimed to investigate viral infections and the prevalence of influenza-like illness（ILI）in Shijiazhuang,China,in 2011and to provide a scientific basis for the diagnosis and control of respiratory tract infections.Throat swab specimens were collected from 483cases of ILI who were outpatients in the influenza surveillance sentinel hospitals in Shijiazhuang between January and December 2011.All specimens were examined by multiplex RT-PCR for the following 15respiratory tract viruses：adenovirus（ADV）,human rhinovirus（HRV）,human parainfluenza virus（PIV types 1-4）,influenza virus A（FluA）,influenza virus B（FluB）,human enterovirus（HEV）,respiratory syncytial virus（RSV-A andB）,human metapneumovirus（HMPV）,human coronavirus（HCoV-229E/NL63and-OC43/HKU1）,and human bocavirus（HBoV）.Among the 483cases of ILI,214（44.31%）were positive for viruses,including ADV（8.7%）,HEV（8.7%）,RSV-A（8.07%）,HRV（7.45%）,FluA（5.38%）,HCoV-OC43/HKU1（2.9%）,PIV-3（2.9%）,HMPV（1.86%）,PIV-1（1.24%）,HCoV-229E/NL63（1.04%）,PIV-2（1.04%）,HBoV（0.83%）,and FluB（0.41%）.Twenty-six（5.38%）of all cases were co-infected with two or more viruses,most commonly HEV/HRV with other viruses.Cases of viral infection were detected throughout the year,with peaks in January and February.ADV and HRV were detected throughout almost the whole year without obvious seasonality.HEV was detected between April and November,with a peak of prevalence in summer and autumn.FluA and FluB reached epidemic levels mainly in winter and spring.All cases of RSV were identified to be subtype A.PIV infection was mainly caused by PIV-3.The positive rate of HCoV-OC43/HKU1infection was significantly higher than that of HCoV-229E/NL63.The leading five viruses that resulted in ILI Shijiazhuang in 2011were HEV,ADV,RSV-A,HRV,and FluA,and these viruses have different epidemiological features.
出处
《病毒学报》
CAS
CSCD
北大核心
2014年第4期391-395,共5页
Chinese Journal of Virology
基金
(艾滋病和病毒性肝炎等重大传染病防治科技重大专项)病毒性传染病病原谱流行规律及变异研究(2013ZX10004202)
关键词
ILI
多重PCR
呼吸道病毒
Influenza-like illness
Multiplex PCR
Respiratory virus
