摘要
目的探讨趋化因子Fractalkine受体CX3CR1基因位点249V/I多态性与早发冠心病以及血脂比值的相关性。方法入选患者分为早发冠心病组（n=149,年龄〈50岁）、晚发冠心病组（n=150,年龄〉65岁）和健康对照组（n=149,年龄47-93岁）,均接受血脂水平检测,计算总胆固醇/高密度脂蛋白胆固醇比值（TC/HDLC）、载脂蛋白B/载脂蛋白A1比值（ApoB/ApoA1）,应用聚合酶链反应和限制性片段长度多态性方法对CX3CR1基因位点249V/I多态性分布进行分析,比较CX3CR1基因多态性及血脂比值在三组之间的差异性。结果等位基因I249在三组的分布频率比较差异有统计学意义（P〈0.0001）;早发冠心病组TC/HDLC和ApoB/ApoA1比值明显高于晚发冠心病组（P〈0.0001）,且独立于249V/I基因多态变异。结论 CX3CR1等位基因I249变异与冠心病的发病年龄存在相关性。高血脂比值与冠心病的发病年龄存在相关性。
Aim To investigate the association between Fractalkine receptor CX3CR1 gene 249V/I polymorphism and early-onset coronary artery disease（ CAD） and lipid ratios in patients with no classic risk fators. Methods By polymerase chain reaction and restriction fragment length polymorphism（ PCR-RFLP）,we examined the frequencies of V249 I among early-onset CAD patients（ n = 149; 50 years）,late-onset CAD patients（ n = 150; 65 years） and healthy controls（ n = 149,47 ~ 93 years） without known CAD risk factors. We compared plasma total cholesterol（ TC） /high density lipoprotein cholesterol（ HDLC） ratio and apolipoprotein B（ ApoB） /apolipoprotein A1（ ApoA1） ratio among the groups and mutation carriers and non-carriers. Results There were significant differences in 249 V /I allele frequencies among the three groups（ P〈0. 0001）. TC /HDLC and ApoB /ApoA1 ratios were significantly higher in early-onset CAD patients compared with late-onset CAD patients and healthy controls（ P〈0. 0001） and independent on either mutation. Conclusions The I249 allele of the Fractalkine receptor CX3CR1 gene may be associated with the age of patients with CAD. We also found an independent association between high lipid ratios and the age of patients with CAD.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2014年第8期827-830,共4页
Chinese Journal of Arteriosclerosis
