摘要
在盐度为0、10、20、30时测定了萨罗罗非鱼(Sarotherodon melanotheron)、尼罗罗非鱼(Oreochromis niloticus)和以色列红罗非鱼(Oreochromis sp.)鳃和肾中Na+-K+-ATPase活性。结果表明:(1)不同盐度对不同组织的Na+-K+-ATPase活性有显著影响,鱼的品种及其他交互作用对Na+-K+-ATPase活性均无显著影响;(2)在实验盐度范围内,无论是鳃和肾,萨罗罗非鱼、尼罗罗非鱼和以色列红罗非鱼的Na+-K+-ATPase活性均随盐度的升高而增高;3种罗非鱼中,萨罗罗非鱼的Na+-K+-ATPase活性随盐度的升高增大最为剧烈,以色列红罗非鱼次之,尼罗罗非鱼最小;低盐度时尼罗罗非鱼的Na+-K+-ATPase活性相对较高,高盐度时萨罗罗非鱼的Na+-K+-ATPase活性相对较高,Na+-K+-ATPase的活性与罗非鱼的耐盐能力有着一定的联系;(3)盐度大于7.19和11.94时,萨罗罗非鱼鳃中的Na+-K+-ATPase活性分别开始高于以色列红罗非鱼和尼罗罗非鱼;盐度大于18.42时,萨罗罗非鱼肾中的Na+-K+-ATPase活性开始高于尼罗罗非鱼;(4)除了在盐度20和30中的尼罗罗非鱼及盐度30的以色列红罗非鱼,是肾中的Na+-K+-ATPase活性高于鳃外,其他均为鳃中的Na+-K+-ATPase活性高于肾,肾中Na+-K+-ATPase活性在不同盐度之间的变化较鳃剧烈;罗非鱼的鳃比肾在渗透压调节上起的作用更大。
This study focused on the effects of salinity on Na＋-K＋-ATPase activities in gills and kidneys of Blackchin tilapia（Sarotherodon melanotheron）,Nile tilapia （Oreochromis niloticus）and Israel red tilapia （Oreo-chromis sp.）.Na＋-K＋-ATPase activities at different salinities（0,10,20 and 30）were measured with ATPase test kit.The results were as follows：（1 ）Salinity significantly affected the Na＋-K＋-ATPase activities of different tis-sues,fish species and interactions between different treatments had no significant effect on Na＋-K＋-ATPase activi-ty;（2）Na＋-K＋-ATPase activities in gills and kidneys of Blackchin tilapia,Nile tilapia and Israel red tilapia all increased with the rise of salinity,among which Na＋-K＋-ATPase activity of Blackchin tilapia rose sharply ,fol-lowed by Israel red tilapia and Nile tilapia.Na＋-K＋-ATPase activity of Nile tilapia was relatively stronger at low salinity,while the activity of Na＋-K＋-ATPase for blackchin tilapia was relatively stronger at high salinity,indica-ting that the Na＋-K＋-ATPase activity of tilapia was related to their salt tolerance;（3 ）Na＋-K＋-ATPase activity in gills of Blackchin tilapia was higher than that of Israel red tilapia when the salinity was above 7.19,and higher than that of Nile tilapia when the salinity was above 1 1 .94.Na＋-K＋-ATPase activity in kidneys of Blackchin tilapia was higher than that of Nile tilapia when the salinity was above 18.42;（4）Except for Nile tilapia at the salinities of 20 and 30,and Israel red tilapia at the salinity of 30,Na＋-K＋-ATPase activities in gills of the three species wese all higher than those of in kidneys,the reaction of Na＋-K＋-ATPase activity in kidney was stronger than in gill,indica-ting that the gill of tilapias was much more responsible for osmoregulation than kidney.
出处
《水生态学杂志》
北大核心
2014年第4期68-73,共6页
Journal of Hydroecology
基金
国家自然科学基金项目(30972252)
河南省重点科技攻关项目(122102310357)
河南省水产学省级重点学科资助
