摘要
目的：探讨CD8＋CD28-T细胞在子宫内膜异位症（ EMT）发病机制中的作用。方法收集EMT患者合并盆腔积液80例（Ⅰ期15例、Ⅱ期12例、Ⅲ期22例、Ⅳ期31例），采用流式细胞术检测其外周血及盆腔积液内CD8＋CD28-T细胞及其胞内细胞因子转化生长因子β1（TGF-β1）及白介素10（IL-10）比率，并选取同期年龄相似的20例健康体检女性志愿者为对照组。结果与健康对照组比较，Ⅰ-Ⅳ期EMT患者外周血CD8＋CD28-T细胞、TGF-β1及IL-10比率均明显升高，表现为Ⅳ＞Ⅲ＞Ⅱ＞Ⅰ，且两两比较差异均有统计学意义（ P ＜0.05）；EMT患者盆腔积液CD8＋CD28-T细胞、TGF-β1及IL-10的频率同样表现为Ⅳ＞Ⅲ＞Ⅱ＞Ⅰ，且以TGF-β1的组间差异最为明显（ P ＜0.05）。 CD8＋CD28-T细胞、TGF-β1及IL-10与分期呈正相关（ rs ＝0.791，0.753，0.726， P ＜0.05）。结论 CD8＋CD28-T细胞、TGF-β1及IL-10在EMT发病机制当中具有重要作用，三者可能是EMT的一个不利因素，并且与EMT的分期密切相关；细胞因子TGF-β1在EMT盆腔积液形成过程中起到重要作用。
Objective To explore the expression and clinical significance of CD 8 ＋CD28 -T lymphocytes in the patients with endometriosis （ EMT） complicating with pelvic fluid .Methods Eighty patients with endometriosis complicating with pelvic fluid （ ca-ses of ⅠtoⅣwere 15, 12, 22, and 31, respectively） were enrolled.The peripheral blood as well as the pelvic fluid were collected and flow cytometry was applied to detect the frequencies of CD 8 ＋CD28 -T lymphocytes and their intracellular cytokines , including transforming growth factor β1 （TGF-β1） and interleukin 10 （IL-10）.Twenty health women with the same range of age were enrolled as the control group .Results Compared with the control ones , the frequencies of peripheral blood CD 8 ＋CD28 -T lymphocytes , TGF-β1, and IL-10 of the EMT subjects were increased significantly （all P 〈0.05）, which presented the order Ⅳ〉Ⅲ〉Ⅱ〉Ⅰand the difference between every two of the Ⅰ-Ⅳgroups was significant （all P 〈0.05）.Similarly, the data of CD8 ＋CD28 -T lymphocytes, TGF-β1, and IL-10 in pelvic fluid also presented as Ⅳ〉Ⅲ〉Ⅱ〉Ⅰ.Moreover, the difference of pelvic fluid TGF-β1 between any two groups was more apparent compared with CD 8 ＋CD28 -T lymphocytes and IL-10 （ P 〈0.05 ） .Spearman correlation analysis showed that any one of CD8 ＋CD28 -T lymphocytes, TGF-β1, and IL-10 positively correlated with any stage of ⅠtoⅣ（ rs =0.791, 0.753,0.726 and all P 〈0.05）.Conclusions CD8 ＋CD28 -T lymphocytes, TGF-β1, and IL-10 were closely related to the stages and could be the negative roles in the pathogenesis of EMT .TGF-β1 played a vital role in the formation of pelvic fluid .
出处
《中国医师杂志》
CAS
2014年第6期770-773,共4页
Journal of Chinese Physician
