肝细胞生长因子对肺癌细胞血管外游走的影响被引量：14

The effect of hepatocyte growth factor on extravasation migration of lung cancer cells

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摘要目的 :评价肝细胞生长因子 ( HGF)对肺癌细胞血管外游走的影响。方法 :利用体外癌细胞血管外游走模型 ,观察 VMRC- LCD、LK- 2、RERF- LC- KJ等 3种肺癌细胞的血管外游走状况 ,并计算游走至血管外的肺癌细胞数。结果 :LK- 2和 RERF- LC- KJ可以通过血管内皮细胞间缝隙游走至血管外、肝细胞生长因子能够刺激肺癌细胞更多地浸润至血管外基质。结论 :肝细胞生长因子能增强肺癌细胞的运动能力。 Objective:To evaluate the effect of hepatocyte growth factor on extravasation migration of lung cancer cells. Methods: Observe extravasation migration of lung cancer cells (VMRC LCD,LK 2,RERF LC KJ) and figure out a count of lung cancer cells beneath the HUVEC monolayer (migrated lung cancer cells) using an vitro model of cancer cell transmigration across a monolayer of HUVEC cultured on collagen gel.Results: Lung cancer cells (LK 2 and RERF LC KJ) cought migrate through a gap between adjacent endothelial cells into collagen gel, A number of transmigrated lung cancer cells(LK 2 and RERF LC KJ) was found before treatment with hepatocyte growth factor. Conclusion: Hepatocyte growth factor as a motogen may stimulate extravasation migration of the lung cancer cell.

作者辛华郑雅娟韩振国闫继东王岩

机构地区吉林大学中日联谊医院胸外科吉林大学第二医院眼科公主岭市医院

出处《吉林大学学报（医学版）》 CAS CSCD 北大核心 2002年第4期360-362,共3页 Journal of Jilin University：Medicine Edition

关键词肝细胞生长因子肺癌转移血管外游走肿瘤转移 hepatocyte growth factor lung cancer metastases extravasation migration

分类号 R734.2 [医药卫生—肿瘤] R73－37 [医药卫生—肿瘤]

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1Praveena SK,Zarnegar R,Siegfried JM. Stimulatory effects of hepatocyte growth factor on normal and neoplastic human bronchial epithelial cells[J]. Am J Physiol,1995,268:1012-1020.
2Zetter BR. The cellular basis of site-specific tumor metastasis[J]. New England J Med,1990,322:605-611.
3Han ZG. Regulation of motility, invasiveness and growth of lung cancer cells by hepatocyte growth factor/scatter factor[J]. Surg Res Comm,1996,18:217.
4Takao N, Kunio M, Akira K. Induction of hepatocyte growth factor in fibroblasts by Tumor-derived factors affects invasive growth of tumor cells:in vitro analysis of tumor-stromal interactions[J]. Cancer Research,1997,57:3305-3313.
5Jiang WG, Grimshaw D, Lane J. A hammerhead ribozyme suppresses expression of hepatocyte growth factor/scatter factor receptor c-MET and reduces migration and invasiveness of breast cancer cells[J]. Clin Cancer Res, 2001, 7: 2555-2562.
6Zetter BR. Adhesion molecules in tumor metastasis[J]. Semin Cell Biol,1993,4:219-229.
7Zarnegar R,Defrancis MC.Expression of HGF-SF in normal and malignant human tissues[J].Exper Suppl,1993,65:181-199.
8Schmidt NO,Westphal M,Hagel C. Levels of vascular endothelial growth factor, hepatocyte growth factor/ scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis[J]. Int J Cancer,1999,84:10-18.