摘要
碳酸亚乙烯酯 (VCA)分子中的反应性环碳酸酯基可于温和条件下与氨基发生反应形成稳定的氨基甲酸酯 .利用这一性质 ,将含— NH2 基团的酶分子直接以σ键的形式固定于含有环碳酸酯基的聚合物载体上 .本文通过反相悬浮聚合 ,以液体石蜡为介质 ,VCA为反应性单体 ,甲基丙烯酸 -β-羟乙酯 (HEMA)及丙烯酸羟丙酯 (HPA)为亲水性共聚单体 ,合成出一系列交联树脂聚合物 .以此聚合物为载体对葡萄糖淀粉酶进行固载实验 ,表现出良好的固定化性能 .同时 ,固定化酶的稳定性也有所提高 .
Functional cyclic carbonate groups can react easily with amino groups under mild conditions without activation. New polymer supports from VCA and other hydrophilic comonomers were prepared in paraffin oil by means of reverse phase suspension polymerization. Then these reactive, microspheric beads were used as matrix for glucoamylase immobilization and exhibited good properties. In addition, the stability of the fixed enzyme was improved.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2002年第8期1605-1609,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金 (批准号 :5 9973 0 0 9)资助
