摘要
目的研究互隔交链孢酚(AOH)对小鼠胚胎成纤维细胞NIH3T3中DNA聚合酶β(DNAPOLβ)表达的影响。方法采用半定量RT-PCR、细胞免疫化学和Western blot方法检测细胞中DNAPOLβmRNA和蛋白的表达。结果与对照组相比,AOH可以引起NIH3T3细胞中DNAPOLβ基因的mRNA水平和蛋白水平表达增高(并呈一定的剂量效应关系)(P<0.05)。结论AOH可以引起NIH3T3细胞中DNAPOLβ基因表达增高,可能有助于细胞应对AOH引起的DNA损伤。
Objective To study the effects of Alternariol （AOH） on DNA polymerase β （ DNA POLβ） expression in NIH3T3 cells. Methods RT-PCR, Immunocytochemistry and Western blot were used to detected mRNA and the protein levels of DNA POLβ in NIH3T3 cell line induced by AOH. Results The expression of DNA POLβ in NIH3T3 cells contaminated by AOH was significantly higher than that in the control group（ P 〈 0. 05 ）. Conclusion AOH induces overexpression of DNA POLβ in the NIH3T3 Cells, which could be responsible to the DNA damage in cells caused by AOH.
出处
《基础医学与临床》
CSCD
北大核心
2008年第10期1030-1034,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(30471952)
河南省教育厅自然科学基金(2007310020)
