摘要
本实验采用大鼠经额向基底池Willis环处插管注入NPY,NE和5-HT,以γCBF变化为指标,观察它们对脑血管的在体效应。实验发现NPY对脑血管具有很强的收缩作用,以克分子浓度计算NPY引起脑血管收缩比5-HT强10倍,比NE强500倍,并可增加NE,5-HT的缩血管效应。人及大鼠CSF中NPY测定发现:SAH伴有CVS的病人,CSF中NPY达291.1±58pg/ml,与对照组(164±28.00pg/ml)相比,P<0.01;大鼠SAH后CSF中NPY含量从2.1±2.1增至6.0±3.2ng/ml(P<0.01),同时大鼠皮层内NPY含量从9.5±0.8下降至4.8±0.8ng/ml(P<0.01),提示脑神经元分泌的NPY参与了CVS过程,并在维持CVS持续状态方面起重要作用。实验还提示SAH病人CSF中NPY含量变化可以作为观察CVS是否出现的指标。
Neuropeptide Y (NPY) was believed a new potent vasoactive neuropeptide and major cerebral arteries have an abundant supply of NPY nerve fibers. To assess the role of NPY on cerebral vessels and vasospasm after subarachnoid hemorrhage (SAH), the NPY, noradrenaline (NE), serotonin (5HT) or fresh blood were injected into basal cistern around willis cycle to test their effects on cerebral vascular in vivo in rats changes of regional cerebral blood flow (rCBF) was used as an index. The experimental results showed that extravascular administration of NPY caused obvious decreasing γCBF of cortex. On molar basis NPY Was more potent than NE (500 fold) and 5-TH(10 fold). NPY also potentiate the effects of 5-HT and NE. When mixing NPY with 5-TH or NE was injected into basal cistern, the recovery time of γCBF caused by these vasoconstrictors will be very much delayed. The NPY of CSF was determined in patients and rats after SAH. The levels of NPY in patients with cerebral vasospasm (CVS) after SAH (295.1+/-30.6) were higher than control patients (164.5+/-13.5 pg/ml, P<0.01). The levels of NPY were increasd from 2.1+/-2.1 to 6.0 +/-3.2 pg/ml (P<0.05) in CSF but were decrease from 9.5+/-0.8 to 4.8+/-0.8 ng/g in cortex (P<0.01), in the rats after SAH. These results suggested that NPY in brain may take part in CVS and that NPY may play an important role in the maintenance of CVS. These results also suggested that changes of NPY level in CSF of SAH patients can be used as a signal of CVS emerging.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1991年第4期412-416,共5页
Chinese Journal of Pathophysiology
关键词
蛛网膜下腔
神经肽Y
出血
Neuropeptides
Cerebral Vasospasm
Subarachnoid hemorrhage
Serotonin
Noradrenaline
