摘要
本文采用放射性配体结合方法观察大鼠心肌缺血与重灌时α_1肾上腺素受体及其亚型——α_(1a)与α_(1b)改变。结果显示左冠状动脉结扎后4小时,缺血心肌的α_1受体与^(125)I-BE结合的数量与亲和性不变,但由对照时含有α_(1a)与α_(1b)两种亚型转变为只含α_(1b)一种亚型。离体灌流心脏停灌30min后α_1受体及其亚型均无显著改变,当停灌30min后再重灌15min时虽然α_1受体的数量不变,但与^(125)I-BE结合的亲和性下降,同时也发生α_(1a)亚型向。α_(1b)亚型的转换。
The changes of myocardial α_1-adrenoceptor and its subtypes—α_(1α) and α_(1b) during ischemia and reperfusion in rat were observed by method of radioligand binding. The resulte showed that at 4 hours after coronary ligation the quantity and the affinity of α_1-adrenoceptor binding to ^(125)I-BE were not changed in ischemic area. But in the ischemic tissues only one subtype—α_(1b) was left, in contrast to the control tissues in which both subtypes —α_(1a) and α_(1b) existed. In isolated perfusion heart: 30 min suspension of perfusion caused no change of α_1-adrenoceptor and its subtypes. Thirty rain suspension of perfusion following 15 min reperfusin, however, caused decrease of affinity of α_1-adrenoceptor to ^(125)I-BE and turnover from α_(1a) subtypeto α_(1b) subtype.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1991年第2期122-124,共3页
Chinese Journal of Pathophysiology
基金
国家自然科学基金
关键词
心肌缺血
Α1受体
肾上腺素
病理
Receptors, adrenergic, alpha
Myocardium
Ischemia
