摘要
目的:研究清心养神方药对应激大鼠心肌细胞肾上腺素β受体的干预效果。方法:采用放射配基结合法,分析实验心肌细胞肾上腺素β受体亲和力、密度和RT-PCR实验检测心肌细胞肾上腺素β受体mRNA,观察清心养神方药对束缚应激大鼠心肌细胞肾上腺素β受体变化的干预作用,并设心得安和朱砂安神丸组作为对照。结果:清心养神方药、心得安和朱砂安神丸均能增加心肌细胞肾上腺素β受体的亲和性,起到增敏现象;对应激引起的受体密度下降有明显抑制作用;对应激引起的β1-AR mRNA表达下调有抑制作用;可上调应激大鼠β2-AR mRNA表达。结论:清心养神方药具有类心得安样受体阻滞作用,对应激引起的心肌细胞肾上腺素β受体改变具有调节作用。
Objective：To investigate the effects of Qingxin Yangshen Chinese herbs on β-adrenoceptor in cardiocytes of stress rats.Methods：Adopting the method of radoligand binding,the affinity of β-adrenoceptor of experimental myocardium cells and density were analyzed.Meanwhile,RT-PCR was used to test mRNA of β-adrenoceptor of myocardium cells.To observe the effect of Qingxin Yangshen Fang on β-adrenoceptor of myocardium cells of stress rats,and the Zhusha Anshen Pills and Propranololas the control groups.Results：All the three groups above can increase the affinity of β-adrenoceptor of experimental myocardium cells,promote its sensitivity and notably inhibit the decrease of density of receptors and control down-regulation of expression of β1-ARmRNA induced by stress,and regulate up the expression of β2-ARmRNA.Conclusion：The Chinese herbs of Qingxin Yangshen had the effect of acceptor inhibiting and can regulate the changes of β-adrenoceptor of myocardium cells induced by stress.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2010年第5期670-673,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金课题(No.30371771)~~
关键词
清心养神方药
应激
大鼠心肌细胞
肾上腺素β受体
Qingxin Yangshen Chinese herbs
Stress
Myocardium cells of rats
β-adrenoceptor
