摘要
警纹鸣夜蛾颗粒体病毒(简称AeGV)、小菜粉蝶颗粒体病毒(PrGV)、茶蚕颗粒体病毒(AbGV)和分月扇舟蛾颗粒体病毒(CasGV)是在形态结构及生物学特性上有显著差异的颗粒体病毒。本文以这四种颗粒体病毒为代表,用统计学方法分析纯净的颗粒体悬浮液在270nm波长处的浓度(C,;μ/ml)与吸光度(A270nm)的,相关性,探讨分光光度法测量颗粒体悬浮液浓度的共用方程式,所有结论均以95％的概率水平为判断依据。本研究证明:颗粒体浓度在10—50/μg/ml范围内时,不同种的颗粒体浓度-吸光度定量关系可以用同一方程式表达。因此,可将该经验式用于快速测定纯净颗粒体悬浮液的浓度,该公式写作为: A270nm=0.018+0.0109C μg/ml (0.1≤A≤0.6)
Agrotis exclamationis granulosis virus (AeGV), Andraca bipnuctata GV(AbGV), Clostera anastomosis GV(CasGV)and Pieris rapae GV (PrGV) are much different in morphological and biological characteristics. The four viruses were ehesen as the representations of the granulosis viruses in our study. Statistical method was used to analyse the correlation between the absorbanees(A) at the wavelength of 270nm and the eoeentrations(C)of pure granulosis viruses suspensions solutions in order to search for a eommon equation that is able to estimate granules concentration. All of the conclusions were decided to depend on 95% probability. The study showed when the concentrations are in the range from 10 to 50μg/ml, the quantitative relationship between ahsorbanees at 270nm and concentrations of different strains granules is expressed with an alike common equation Thus, this common equation can be used as an experimental equation to calculate the concentration of pure granu- losis virus suspension fastly by spectrophotometry. This empirical fornlula is A_(270nm)=0.018+0.0109Cμg/ml (0.1≤A≤0.6).
出处
《中国病毒学》
CSCD
1991年第3期260-266,共7页
Virologica Sinica
关键词
颗粒体病毒
颗粒体
分光光度法
Granulosis virus Granules Statistical analysis Speetrophotometry Estimation of concentration
