摘要
利用一对特异性引物 ,用PCR的方法从禾谷缢管蚜体内扩增出了病毒结合蛋白基因 ,序列测定结果表明其长度为 1 6 4 7bp ,编码 5 4 8个氨基酸 ,与GenBank中的禾谷缢管蚜美国生物型BuchneragroEL NT核苷酸序列同源性为 97% ,氨基酸同源性为 97 4%。构建了 2个原核表达载体并进行表达得到了 6 9kD融合蛋白和 6 3kD的非融合蛋白。
Viral binding protein gene from Rhopalosiphum padi Yangling biotype was amplified by PCR method and then cloned.The complete nucleotide sequence of the gene was determined,It has 1647 nucleotides encoding 548 amino acids.Comparison showed this gene has 97% identity on nucleotide level with Buchnera groEL AM gene of Rhopalosiphum padi American biotype,while has 97.4% identity on amino acid level was found between this two genes.The VBP gene was ligated into pBV221 and pET30a expression vector and expressed the aim protein 63kD and 69kD.
出处
《微生物学报》
CAS
CSCD
北大核心
2002年第4期448-452,共5页
Acta Microbiologica Sinica
基金
国家自然科学基金 ( 39970 4 83)
国家八六三青年基金 ( 97 1 37)~~
