摘要
目的 :研究一氧化碳及其限速酶 (血红素氧合酶 - 1)对局灶性缺血脑组织血脑屏障通透性的影响。方法 :将SD大鼠随机分为 3组 (n =6 ) ,使用血红素氧合酶诱导剂及抑制剂腹腔注射 ,用等量生理盐水腹腔注射作为对照组 ,12h后复制MCAO模型。梗塞后 2 4h后检测血液中一氧化碳浓度、血脑屏障通透性。结果 :诱导剂组一氧化碳浓度明显高于对照组 (P <0 0 1) ,血脑屏障通透性明显低于对照组 (P <0 0 5 ) ,而抑制剂组一氧化碳浓度明显低于对照组 (P <0 0 1) ,血脑屏障通透性明显高于对照组 (P <0 0 5 )。血红素氧合酶诱导剂、抑制剂对非梗塞侧的血脑屏障通透性没有影响 (P >0 0 5 )。结论 :一氧化碳作为一种信使分子 。
AIM: To evaluate the effect of carbon monoxide(CO) on the permeability of brain blood barrier(BBB) in cerebral ischemic rats. METHODS: SD rats were divided into three groups. Saline, hemin or ZnPP were injected intraperitoneally 12 h before middle cerebral artery occlusion (MCAO), respectively. The concentration of blood CO and the permeability of BBB at 24 h after MCAO were measured. RESULTS: The CO concentration in blood in hemin group was higher than that in saline group( P< 0.01), but those in ZnPP group was lower than that in saline group( P< 0.01). In infracted regions, the permeability of BBB in hemin group was lower than that in saline group, and those in ZnPP group was higher than that in saline group ( P< 0.05). There was no significant changes in BBB permeability among hemin, ZnPP and saline groups in noninfarcted side( P> 0.05). CONCLUSION: CO reduced the permeability of BBB as a messenger gas molecular when its intrinsic concentration was elevated.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第7期798-800,共3页
Chinese Journal of Pathophysiology
基金
复旦大学优秀博士学位论文培育基金资助(No .CQF15 180 1)
关键词
脑缺血
一氧化碳
血红素氧合酶
血脑屏障
渗透性
Brain ischemia
Carbon monoxide
Heme oxygenase
Blood-brain barrier
Permeability
