摘要
目的 :观察蛋白激酶Cα亚型 (PKCα)的反义寡核苷酸对鼻咽癌CNE - 2Z细胞生长、细胞周期和cyclinE表达的影响。方法 :脂质体法转染反义PKCα,MTT法测细胞生长 ,流式细胞仪检测细胞周期 ,免疫细胞化学及点印迹法检测cyclinE表达。结果 :①随着反义PKCα的浓度增加 ,细胞生长指数逐渐降低 (P <0 0 1)。②反义PKCα使细胞G1期百分比增高 (P <0 0 5 )。③反义PKCα使细胞cyclinE的表达减弱 ,扫描定量反义PKCα组为对照组的 6 6 5 %± 18 4 % (P <0 0 5 )。结论 :反义PKCα可通过抑制cyclinE表达、阻滞细胞于G1期从而抑制CNE -
AIM: To observe the effect of protein kinase C-α(PKCα)antisense oligonucleotide on cell growth, cell cycle and the expression of cyclin E in human poor-differentiated nasopharyngeal carcinoma(NPC) cell line CNE-2Z. METHODS: Antisense PKCα was transfected by cationic liposome(LP) in CNE-2Z cells to analyze the cell growth and cell cycle by MTT colorimetric assay and flow cytometry, respectively. Moreover, the expression of cyclin E was determined by immunocellularchemistry and scanning the result of dot-blotting. RESULTS: ①With the concentration of antisense PKCα increasing, the relative cell growth index was decreased gradually( P< 0 01). ②After treated with antisense PKCα, the percentage of cells in G 1 phase enhanced( P< 0 05). ③Compared with the control group, the expressing intensity of cyclin E reduced in antisense PKCα group, and the expression of cyclin E decreased to 66 5%±18 4%( P< 0 05) of the control by scanning quantitative analysis. CONCLUSION: These results indicated that antisense PKCα may inhibit cell growth in CNE-2Z via suppressing the expression of cyclin E and hindering cell process in G 1 phase.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2002年第7期782-784,I002,共4页
Chinese Journal of Pathophysiology
基金
广东省自然科学基金资助项目 (No.95 0 6 6 1)
广东医学院青年基金项目 (No .青 970 5 )
关键词
蛋白激酶C
鼻咽肿瘤
细胞周期
反义寡核苷酸类
Protein kinase C
Nasopharyngeal neoplasms
Cell cycle
Oligonucleotides, antisense
