摘要
繁殖于昆诺阿藜(Chenopodium quinoa)、千日红(Gompherena globosa)及番杏(Tetragonia.expansa)上的病毒分离物,经PEG6000沉淀结合差速离心浓缩后,用Sepharose4B柱层析法进行分离纯化,获得保持侵染活性的病毒纯化物;而且表明,用昆诺阿藜繁殖病毒,效果最优。紫外扫描测定,纯化病毒的最高吸收波长为258nm,最低吸收波长为244nm,A260/A280为1.515。分析超速离心测定,病毒粒体为单一沉降组分,S20.w约73S。SDS-PAGE结果表明,病毒衣壳蛋白亚基由一条分子量为27.88×10~3±2.99×10~3daltons的多肽组成。根据紫外吸收估计,病毒粒体中核酸含量约12—15%;RNaseA酶解及变性处理病毒核酸后琼脂糖变性电泳结果说明,病毒基因组为一条ssRNA。根据该病毒的生物学特性及生化特性,初步确认为一种新的病毒分离物,并定名为中国水仙条纹病毒(Chinese Narcissus Strip Virus,ChNSV),其密码程式为R/1:*/12—15:S/S:S/*。
By differential centrifugation and Sepharose 4B column chromatography, the virus was qurified from the inoculated leaves of propagation hosts of Chenopodium quinoa, Gompherena globosa and Tetragonia expansa. It was shown that C. puinoa was the most efficient host for the virus propagation and purification.The purified virus shared a single sedimenting component of 73S (S20.w) in analytical ultracentrifugation. Its coat protein was made up of only one polypeptide with molecular weight of 27.88K卤2.99K daltons in SDS-PAGE.The viral nucleic acid was composed of one single strand RNA determined by denatural agarose electrophoresis after RNase A digestion and denaturation. The virus contained 12-15%RNA estimated from UV absorption spectrum of max absorption at 258nm and min absorption at 244nm with A260/A280 of 1.515.Based on the biological and biochemical properties, this virus was considered to be a previously undescribed one with a cryptogram of R/1:*/12 -15: S/S:S/*. And it was tentatively named as Chinese Narcissus Strip Virus (ChNSV).
出处
《植物病理学报》
CAS
CSCD
北大核心
1991年第2期101-106,共6页
Acta Phytopathologica Sinica
