摘要
比较了几种水稻抗痕性接种鉴定技术,提出了一种新方法——涂抹接种法。在1—2%的羧甲基纤维素水溶液中混以1—2×10~5/ml浓度的稻瘟病菌孢子,涂抹接种于水稻的嫩叶或穗颈节部,可获得良好的接种效果。苗期接种适用于不同生育期幼嫩叶片,但以2—4叶期为佳。孢子浓度10~5/ml时发病串接近100%。涂抹接种法的发病程度重于喷雾接种法,轻于注射接种法。本法应用于穗瘟接种的效果也良好。涂抹接种法较接近自然发病条件,所用菌量少,侵染率高,是一种良好的接种方法。
A simple and precise new method——smear inoculation for evalu-ating rice blast resistance is presented.In this method,inoculation iscarried out with good results by smearing with brush the mixture of
1—2%CMC(Carboxy Methyl Cellulose)and 1-2x10^5 conidia/ml ontothe tender rice leaf and neck of the rice panicle.The new method issuitable for tender rice leaves at various growing stages,especially,the 2—4 leaf-age seedling.Spore suspension of 1×10~5 conidia/ml in theinoculum suspension is adequate to give nearly 100% leaf infection.Smear inoculation produces higher disease incidence than spraying inoc-ulation,and less incidence than injection inoculation.This new meth-
od is also very useful as a simple and precise method for neck resist-ance evaluation.
Smear inoculation is similar to natural infection,it needs only asmall amount spore suspension,and gives a high infection frequency.
出处
《植物保护学报》
CAS
CSCD
北大核心
1991年第1期53-56,共4页
Journal of Plant Protection
