摘要
稻瘟病菌生理小种的鉴别对培育抗稻瘟病水稻品种具有重要意义.传统方法利用一套鉴别品种区分小种既复杂又易受各种因素的影响.我们在日本稳定菌株北1的基因组文库中筛选出了一套含重复顺序的克隆,2个克隆被证实具有高度的多态性.用其中的POR6对17个稻瘟病菌株进行Southern分析,获得了可分辨和重复的基因组特异的指纹图谱.本文提供了8个稳定的稻瘟病菌株的致病性与其DNA指纹图谱之间关系,结果表明各小种组合间的百分相似率Sxy值与该小种组合间共同侵染的鉴别品种数目有正相关性.
Identification of rice blast fungus is important to breeding blast-resistant rice. The traditional method for assign ment of the blast pathotypes is complicated and its results are vulnerable to the ef-fects of various factors. In order to develop a precise identification method based on DNA fingerprints, a set of clones containing repetitive sequences has been selected from a genomic library which was con-structed from & Japanese pathotype Hoku 1. Two of them were verified to have highly polymorphisms among 17 rice blast isolates. out Southern analysis with POR6 as probe,distinctive and reproducible genome-specific DNA-fingerprints were observed. In this paper,the relationship between pathotypes and DNA-fingerprints of 8 blast isolates is shown. The results indicate that the percentage of similar hybridizing bands (Sxy) in POR6-DNA fingerprints of any two isolates are positively related to the number of rice cultivars which can be infected by both of them.
出处
《作物学报》
CAS
CSCD
北大核心
1994年第3期257-263,共7页
Acta Agronomica Sinica
关键词
稻瘟病菌
DNA指纹分析
分子杂交
Pyricularia oryzae, DNA fingerprint, Probe, Molecular hybridization
