摘要
目的 建立Rh血型系统C/c基因分型的方法 ,检测人群中C/c基因频率。方法 用快速盐析法抽提样本DNA ,用PCR检测C/c基因型。结果 本组RhD阳性人群中 ,C基因型频率为 0 6 74,c基因型频率为 0 32 6。RhD阴性人群中 ,C基因频率为 0 2 34 ,c基因频率为 0 76 6。结论 RhD阳性与阴性人群C和c基因频率存在明显的差异。RhD阳性人群以C为主 ,RhD阴性人群中以c为主。
Objective To establish Rh Cc genotyping method and detect the allele frequencies of Rh Cc in population.Methods Genomic DNA was isolated from buffy coats using fast salting-out method,the Rh Cc genes were typed using polymerase chain reaction.Results The allele frequencies of C and c in Rh D positive population were 0.674 and 0.326 respectively,while the allele frequencies of C and c in the Rh D negative population were 0.234 and 0.766 respectively.Conclusion The differences of the allele frequencies were significant between the Rh D positive and RhD negative population.The major allele is C in the Rh D positive population but c in the Rh D negative population.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2002年第4期204-205,共2页
Chinese Journal of Clinical Laboratory Science
基金
浙江省科委资助项目 ( 99110 3174)
