摘要
A useful helicobacter pylori-induced gastritis model using BALB/c mice was established for mimicking of human gastritis induced by Helicobacter pylori (H. pylori). The H. pylori isolates were obtained freshly from a human complex ulcer patient. BALB/c mice were fasted for 24 h and then 0.25 mL of 0.2 mol·L -1 NaHCO 3 was administered after by gavage to each mouse and 0.5 mL of 10 9 colonies formation unit per milliliter (CFU/mL) of H. pylori was administered 15 min. On the 3 rd day and 5 th day, the H. pylori inoculations were repeated. The inoculated mice were sacrificed in batch on the 5 th day, in the 2 nd week, 3 rd week and 4 th week. The gastric mucous membrane near pyloric portion was removed, treated and then cultured under microaerobic condition for detection of H.pylori. The remainders of the gastric membrane were fixed by 10% formaldehyde solution for pathological detection. The results showed that the H. pylori could be separated from the gastric membranes of inoculated mice. Obvious invasion of inflammatory cells in the gastric membranes of inoculated mice could be observed from pathological sections. It can be concluded that the inoculating fresh human H. pylori isolates can produce mouse gastritis. This model of BALB/c mice can be used for evaluating the therapeutic agents for the treatment of gastritis induced by H. pylori.
本研究的目的在于用人类复合性溃疡患者幽门螺杆菌新鲜分离株感染BABL c小鼠建立动物胃炎模型。幽门螺杆菌 (H pylori)是由Warren和Marshall在 1989年首次发现的 ,此杆菌与胃炎和消化性溃疡相关联 ,从而彻底改变了对胃、十二指肠疾病的认识。 1990年澳大利亚国际胃肠病学术会议确认H pylori是导致胃炎的重要病因 ,由此引起的胃炎是发病率最高的消化性疾病 ,但仍有许多方面了解不够或有不同认识 ,存在的主要问题是缺乏简单、易控制的动物模型。我们选用复合性溃疡患者的幽门螺杆菌临床新鲜分离株 ,在微需氧条件下进行培养传代 ,然后按照 10 9集落形成单位 mL的菌量接种BALB c小鼠 ,接种前将小鼠禁食 2 4小时 ,并用 0 2 5mL 10 %NaHCO3 稍微中和胃部酸性 ,15分钟后接种 ,并于第 3、5天分别重复接种。于最后一次接种后 5天 ,2 ,3,4周分批处死动物 ,取出小鼠接近幽门部的胃粘膜组织 ,一部分用于H pylori鉴定 ,另一部分用于病理学检测。结果表明 ,接种幽门螺杆菌的实验组小鼠胃粘膜能分离出幽门螺杆菌 ,病理切片显示实验组小鼠胃粘膜有不同程度的胃炎 ,而空白对照组小鼠则无明显的炎症表现。从而建立了用人类复合性溃疡患者幽门螺杆菌新鲜分离株感染BALB c小鼠的动物胃炎模型。
