摘要
目的:探讨辅助性T细胞17(Th17)及IL-17在支气管哮喘小鼠中的变化及意义。方法:将24只BABL/c小鼠随机分为哮喘组(12只)和正常对照组(12只)。用卵清白蛋白(OVA)致敏和激发建立小鼠哮喘模型;行苏木精-伊红(HE)染色,观察肺组织炎症改变;流式细胞术检测脾脏单个核细胞中Th17细胞比例;酶联免疫吸附法(ELISA)检测支气管肺泡灌洗液(BALF)中Th17细胞相关细胞因子IL-17水平;并计数BALF中细胞总数、嗜酸粒细胞及中性粒细胞数。结果:哮喘组肺组织及气道周围的炎症改变较正常对照组显著增强。哮喘组脾脏中Th17细胞及BALF中IL-17水平均显著高于正常对照组(P均<0.01),哮喘组BALF中细胞总数及嗜酸粒细胞计数显著高于正常对照组(P均<0.01)。结论:哮喘小鼠存在Th17细胞数量增多及其分泌的IL-17水平增高,IL-17可能通过促进嗜酸粒细胞在气道的聚集而参与炎症反应,Th17细胞可能在哮喘的发病中具有重要作用。
Objective：To investigate the changes of T helper 17 cells and the level of IL-17 in bronchial asthmatic mice. Methods： Twenty-four BABL/c mice were randomly divided into two groups, asthma group and control group. The asthmatic mouse models were established after ovalbumin（OVA） sensitization and challenge. The airway inflammation of lung tissue was observed by hematoxylin and eosin （HE） staining. The ratio of Th17 cells to mononuclear cells in the spleen was detected by flow cytometry. The level of IL-17 in bronchoalveolar lavage fluid（BALF） was measured by enzyme-linked immunosorbent assay（ELISA）. The cell numbers in BALF were counted as total cells,eosinophils and neutrophils. Results：Elevated inflammation were observed in the lung and airway tissues of asthmatic mice when compared with controls. Not only the frequency of Th17 cells in spleen but also the level of IL-17 in BALF of asthmatic mice were significantly higher than those of normal controls （all P 〈 0.01）. The numbers of total cells and eosinophils in BALF of asthmatic mice were significantly higher than those of normal controls（all P 〈 0.01 ）. Conclusion：The study showed that the increasing number of Th17 cells in spleen and level of cytokine IL-17 in BALF are characteristic features in asthmatic mouse model. IL-17 may be associated with the recruitment of eosinophils into airways in asthma.These results demonstrate that Th17 cells may play an important role in the pathogenesis of asthma.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第8期1095-1098,1123,共5页
Journal of Nanjing Medical University(Natural Sciences)
