摘要
选取 2 3个品种 (系 )及 8个杂交组合F1的幼胚作为供试材料 ,取 12～ 16日龄的幼胚接种在附加 2 ,4 -D2 0mg/L和 6 -BA 1 0mg/L的MS培养基上 ,诱导 3～ 5d后所有供试材料都具有愈伤组织。筛选出适宜的继代培养基为 :MS +2 ,4 -D 4 0mg/L +NAA 0 5mg/L +LH 5 0 0mg/L +0 5 %蔗糖 +0 85 %琼脂 ;大穗 2、昌乐 2号、抗锈 2 0 4、品系 38等材料愈伤组织生长较好。植株再生适宜的培养基为 1/2MS +NAA 0 5mg/L +6 -BA0 5mg/L +0 3%蔗糖 +0 85 %琼脂。抗锈 2 0 4、昌乐 2号、优 2 6 5 /昌乐 2号、优 2 6 5 /京选 6号、优 2 6 5 /有芒白 4号等 5个供试材料分化率较高 ;杂交胚与常规品种比较 。
varieties of winter wheat and 8 hybrids were used as testing materials Immature embryos were cultured on MS medium containing BA 1 mg/L, 2,4-D 2 mg/L The results showed that calli formed from embryos of all varieties and hybrids 3～5 days after induction The best subculture medium contained 2,4-D 4 mg/L, NAA 0 5 mg/L, LH 500 mg/L in MS medium Calli from 'Dasui 2', 'Kangxiu 204', 'changle 2' and 'Pinxi 38' was more compact in structure and grew more quickly than other varieties Hybrid calli showed heterosis in growing The best medium for regeneration contained NAA 0 5 mg/L, BA 0 5 mg/L in 1/2 MS The calli differentiation rate of 'Changle 2', 'Kangxiu 204', 'You 265/Jingxuan 6', 'You 265/Youmangbai 4', 'You 265/Changle 2' was higher than that of other varieties Plant differentiation rate of hybrids calli also showed heterosis
出处
《河北职业技术师范学院学报》
2002年第2期1-5,11,共6页
Journal of Hebei Vocation-Technical Teachers College
基金
河北省自然科学基金课题 (课题编号 :30 12 95 )部分内容
