摘要
以Tagsk1(TriticumasetiumL.glycogen synthase kinase1)基因的cDNA的碱基序列为基础,设计特异引物由小麦耐盐突变体RH8706-49基因组DNA进行扩增后,得到来自于基因组的Tagsk1基因。采用基因枪法,利用携带该基因的双元表达载体pBI121-gsk1转化敏盐小麦H8706-34和中国春的成熟胚愈伤组织,经Kanamycin和0.5%NaCl筛选获得耐盐愈伤组织。这些被转化的愈伤组织表现出较高的耐盐性,并且能够在含盐培养基上进一步分化出根和芽。
The Tagsk1（ Triticum asetium L. glycogen synthase kinase 1）gene derived from the genome of wheat salt-tolerance mutant RH8706-49 was cloned by PCR. The special primers designed according to full length cDNA sequence of Tagsk1 （AF525086）. A binary expression vector pBI121-gsk1 containing Gus and Tagsk1 was constructed, And pBI121-gsk1 was introduced into the callus induced from mature embryos of salt-sensitive wheat H8706-34 and cv. China Spring by particle bombardment. The transformed callus were screened by Kanamycin and 0.5 % NaCl, The salt-tolerance callus were obtained, which showed higher ability of salt-tolerance and could diffirentiate roots and buds on the medium containing 0.5 % NaCl.
出处
《生物工程学报》
CAS
CSCD
北大核心
2006年第2期211-214,共4页
Chinese Journal of Biotechnology
基金
国家转基因专项(No.2004JYA01)
国家自然科学基金(No.30070471)
河北省自然科学基金(No.301103
No.2005000164)。~~
关键词
基因枪
转化
小麦
成熟胚愈伤组织
Tagsk1
microparticle bombardment, transformation, common wheat, callus of mature embryos, Tagsk 1
