摘要
本文用动脉平滑肌细胞(SMC)为实验模型,观察硝苯啶(Nifedipine)对SMC增殖的影响,以探讨它抗动脉粥样硬化(AS)的机理.结果显示:在10%人血清、人高脂血清低密度脂蛋白(LDL)和胰岛素等促SMC增殖因素的作用下,硝苯啶显示了明显的抑制SMC增殖的作用,且抑制作用的时间持久.这一结果提示,硝苯啶可能通过干扰动脉SMC的增殖而有防治人类AS的作用.
Human fetal and rabbit aortic smooth muscle cells (HSMC and RSMC) were successfully cultured. The effect of nifedipine on HSMC and RSMC proliferation was observed by ~3H-thymidine incorporated into the DNA of SMC. The DNA synthesis of HSMC was found to be significantly inhibited at 10^(-7)mol/L nifedipine in serum-containing medium (P<0.05 vs. control),at 10^(-6)mol/L nifedipine in LDL-containing medium (P<0.01 vs.control);and the DNA synthesis of RSMC was inhibited at 10^(-6)mol/L nifedipine in insulin-or LDL-containing medium (P<0.05 or<0.01 vs. control).
关键词
硝苯啶
药理学
血管平滑肌
Calcium Channel Blockers/pharmacol
Nifedipine/pharmacol
Atherosclerosis/etiol
Muscle, Smooth, Vascular/physiopathol
