摘要
目的血管的老化与血管平滑肌细胞（ｖａｓｃｕｌａｒｓｍｏｏｔｈｍｕｓｃｌｅｃｅｌ，ＶＳＭＣ）的异常增殖有关，我们拟探讨其增殖机制并寻求有效防治措施。方法应用噻唑蓝（ｔｈｉａｚｏｌｂｌｕｅ，ＭＴＴ）染色法和３Ｈ－ＴｄＲ掺入法观察去甲肾上腺素（ｎｏｒｅｐｉｎｅｐｈｒｉｎｅ，ＮＥ）、硝苯啶（ｎｉｆｅｄｉｐｉｎｅ，Ｎｉｆ）以及人参皂甙（ｇｉｎ－ｓｅｎｏｓｉｄｅｓ，Ｇｉｎ）对老年（１８月龄）和青年（３月龄）大鼠培养的主动脉平滑肌细胞（ａｏｒｔｉｃｓｍｏｏｔｈｍｕｓｃｌｅｃｅｌ，ＡＳＭＣ）增殖和细胞内ＤＮＡ合成的影响。结果（０．１～５）×１０－５ｍｏｌ／Ｌ的ＮＥ可明显促进ＡＳＭＣ呈剂量依赖性增殖，且在老年鼠明显强于青年鼠（Ｐ＜０．０５）；１×１０－５ｍｏｌ／ＬＮｉｆ可明显抑制ＮＥ（１×１０－５ｍｏｌ／Ｌ）引起的老年鼠ＡＳＭＣ的增殖和细胞内ＤＮＡ的合成（Ｐ＜０．０５），对青年鼠效果不明显；１００ｍｇ／ＬＧｉｎ可明显抑制老年鼠ＡＳＭＣ的增殖和细胞内ＤＮＡ合成（Ｐ＜０．０５），对青年鼠无明显抑制作用。结论老年鼠ＡＳＭＣ较青年鼠更易增殖，两者增殖的机制不同，老年鼠ＡＳＭＣ增殖的机制与细胞钙运转的关系密切。钙离子拮抗剂及人参皂甙可?
Objective Vascular aging is related to abnormal proliferation of the vascular smooth muscle cells (VSMC). The aim was to study VSMC proliferative mechanism and find some effective drugs in prevention. Methods Cell Titer 96 TM Non radioactive cell proliferation assay method and 3 H TdR incorporation method were used to study the effects of norepinephrine(NE), ginsenosides (Gin) and nifedipine (Nif) on the proliferation and DNA synthesis in aortic smooth muscle cells (ASMC) from both 18 months and 3 months old rats. Results NE at doses (mol/L) of 0 1×10 5 ～5×10 5 promoted significantly the proliferation in ASMC from old rats( P <0 05 vs young rats); Nif(10 5 mol/L)inhibited the proliferation and DNA synthesis induced by 10 5 mol/L NE in ASMC from old rats ( P <0 05); Gin (100mg/L) inhibited the proliferation and DNA synthesis in ASMC from old rats. ( P <0 01 vs young rats). Conclusions ASMC is easier to proliferate in old rat than in young rat, and mechanisms are different. In old rat, it is closely related to the calcium transportation. Calcium antagonist Nif and Gin may be the effective drugs to prevent ASMC from proliferation with aging.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
1997年第1期16-19,共4页
Chinese Journal of Geriatrics
关键词
平滑肌
细胞
去甲肾上腺素
硝苯啶
muscle
smooth
vascular
cells
cultured
norepinephrine
nifedipine
ginsenosides
