摘要
给单侧肾切除雄性 Wistar大鼠的阴茎背静脉注射 3 5mg/kg链脲霉素引发糖尿病 ,在 5个月的实验过程中 ,大鼠的血糖水平一直处于稳定的中、高度状态。在实验第 1周大鼠体重明显下降 ,以后体重不再下降 ,也不增重 ,而对照组大鼠在 5个月的实验过程中体重持续增加。实验开始 5个月后 ,糖尿病大鼠的平均动脉压和肾脏血管阻力均显著高于对照组( P<0 .0 0 1 ) ,肾脏血浆流量则显著低于对照组 ( P<0 .0 1 ) ,导致滤过分数显著上升 ( P<0 .0 0 1 ) ,糖尿病大鼠的尿量比对照组明显增加 ,实验 4周起 2 4h的蛋白尿就有显著增加 ( 3 6.8± 3 .6mg vs.2 1 .5± 1 .7mg,P<0 .0 1 ) ,并且随着时间的延续呈进行性升高。无论是心脏与体重比 ( 0 .3 5%± 0 .0 1 % vs.0 .2 4%± 0 .0 1 % ,P<0 .0 0 1 ) ,还是左肾与体重比 ( 0 .99%± 0 .0 7% vs. 0 .3 9%± 0 .0 1 % ,P<0 .0 0 1 ) ,糖尿病大鼠都显著高于对照组。结果提示 ,给单侧肾切除的大鼠静脉注射低剂量 STZ可以诱发糖尿病性肾病模型 ,具有高血压、进行性蛋白尿、肾功能不良的体征。
Type Ⅰ diabetes mellitus were made in Wistar rats by intravenous injection of 35 mg/kg streptozotocin freshly dissolved in citrate-buffered saline solution. Stable moderate hyperglycemia was maintained throughout the duration of the experiment, without receiving any insulin injection. Body weights were markedly reduced at 1 week after induction of the diabetes and maintained throughout the experiment in the untreated diabetic rats, whereas non-diabetic control rats gained weight during the 5-month follow-up. Blood pressure (BP) and renal function data at 5 months after induction of diabetes were summarized. The untreated uninephrectomized diabetic rats developed hypertension with mean arterial pressure (MAP) of 128±2 mmHg under anesthesia. Renal vascular resistance (RVR) was markedly elevated in the untreated diabetic rats as compared to that in controls ( P <0.001), leading to a significant lower renal plasma flow (RPF) ( P <0.01). Glomerular filtration rate (GFR) was comparable between untreated diabetic rats and controls. Thus, Filtration fraction (FF) rose significantly ( P <0.001). Urine flow rate was also markedly increased in the untreated diabetic rats. Untreated diabetic rats showed a significant increase in urinary protein excretion as early as 4 weeks after induction of the disease (36.8±3.6 vs. 21.5 ±1.7, P <0.01 in control rats at week 4), with a substantial progressive increase of this value over time. A ratio of the heart weight to the body weight was 3.5 ±0.1 to 2.4 ±0.1 ( P <0.001) and the left kidney weight to the body weight was 9.9± 0.7 to 3.9 ±0.1 ( P <0.001). Both ratios were markedly higher in untreated diabetic rats as compared to those in controls. Thus, the STZ-injected uninephrectomized rats developed overt diabetic nephropathy, as evidenced by the development of hypertension, progressive proteinuria and renal dysfunction.
出处
《上海实验动物科学》
2002年第2期78-82,88,共6页
Shanghai Laboratory Animal Science
关键词
高血糖
蛋白尿
大鼠
糖尿病性肾病
模型
Wistar rats
Type Ⅰ diabetes mellitus
Nephropathy
Hyperglycemia
Urinary protein
