摘要
对1株黑曲霉产菊粉酶的发酵条件进行了研究,确定了优化的发酵条件为菊粉2%,酵母膏2%,(NH4)H2PO40 5%,Na Cl0 5%,MgSO4·7H2O0 05%,ZnSO4·7H2O0 01%,初始pH6 5,接种量1 6%,装液量30ml,发酵5d,菊粉酶活最高可达26U/ml,I/S为1 15。经Co60诱变筛选出1株突变株C-32,在相同的发酵条件下菊粉酶活提高30%,I/S不变。
The optimum fermentation condition for higher inulinase production of Aspergillus niger was determined as shown below:inulin 2%;yeast extract 2%;NH4H2PO4 05%;NaCl 05%;MgSO4·7H2O 005%;ZnSO4·7H2O 001%;pH65;inoculation 16%Maximal inulinase yield of 26U/ml was obtained after 120h of growth in shake cultures (30ml of medium in 250-ml flasks) and the value of I/S was 1.15.A mutant was isolated after treatment by Co60 .The inulinase activity was enchanced by 30% and the value of I/S was not changed.
出处
《生物技术》
CAS
CSCD
2002年第3期8-9,共2页
Biotechnology
关键词
黑曲霉
产菊粉酶
发酵条件
优化
诱变育种
Aspergillus niger
inulinase
fermentation condition
mutation
