摘要
目的 :研究肺癌患者血清中可溶性Fas(sFas)水平及增殖细胞核抗原 (PCNA)在肺癌组织中的表达及其临床意义。方法 :应用双抗体夹心ELISA方法检测血清sFas含量 ,免疫组织化学链酶亲和素 生物素 过氧化物酶复合物 (SABC)法检测肺癌组织中PCNA的表达 ,并探讨二者与肿瘤发生及恶性行为之间的关系。结果 :与正常人相比 ,肺癌患者血清sFas水平明显升高 (P <0 0 1) ,血清sFas含量和肺癌组织PCNA标记指数均随组织学分级和病理分期的增高而增高 (P <0 0 1) ,有淋巴结转移者较无淋巴结转移者显著增高 (P <0 0 1)。结论 :血清sFas含量和肿瘤组织中PCNA的表达与肿瘤的发生及恶性行为密切相关。
Objective:To study serum soluble Fas(sFas) level and the expression of proliferating cell nuclear antigen (PCNA) in lung cancer and the clinical significance. Methods: Serum sFas level was measured by enzyme linked immunosorbent assay(ELISA). Immunohistochemical streptavidin biotin peroxidase complex (SABC) method was used to detect PCNA expression in lung cancer tissue. Results: Serum sFas level of lung cancer patients was significantly higher than that of normal persons(P<0 01). There was a positive correlation between serum sFas level, PCNA lable index(PCNA LI) and tumor histologic grade, pathological phase and lymph node metastasis (P<0 01). Conclusion: Serum sFas level and the expression of PCNA in lung cancer were significantly related to the growth and malignant behavior of lung cancer.
出处
《贵阳医学院学报》
CAS
2002年第3期206-208,共3页
Journal of Guiyang Medical College
关键词
肺肿瘤
酶联免疫吸附测定
可溶性FAS
增殖细胞核抗原
PCNA
lung neoplasms
carcinoma
enzyme linksed immunosorbent assay
soluble Fas
proliferating cell nuclear antigen
