摘要
利用非共价分子印迹法 ,采用 4 乙烯基吡啶为功能单体 ,以二甲基丙烯酸乙二醇酯为交联剂 ,在模板分子(S) 酮洛芬的存在下 ,制备出 (S) 酮洛芬的分子印迹聚合物 (MIPs) ,并用作HPLC固定相 ,对其进行了高效液相色谱评价 .结果表明外消旋酮洛芬在制备的印迹柱上得到了有效的分离 ,(S) 酮洛芬的容量因子kS′为 9.5 2 ,选择性因子α为 1 5 2 ,分离度R为 0 88.此外 ,HPLC分析表明制备的MIPs能够分离结构相似的酮洛芬、布洛芬和萘普生 .研究了流动相中乙酸浓度、流速及温度对拆分效果的影响 ,测定了分离过程中的焓变、熵变和自由能变化 。
A chiral stationary phase for the enantioselective separation of ketoprofen has been prepared by molecular imprinting, in which 4-vinylpyridine (functional monomer) and ethylene glycol dimethacrylate were copolymerised in the presence of W-ketoprofen. Racemic ketoprofen was efficiently resolved on the molecular imprinting polymer. Capacity factor (k(s)') of (S)-ketoprofen and separation factor (alpha) were 9.52 and 1.52, respectively. Furthermore, the polymer was able to separate ketoprofen from the structurally related ibuprofen and naproxen. Effects of concentration of acetic acid in the mobile phase, flow rate and temperature were studied. The change of enthalpy and entropy were estimated. It was found that enantioselective separation was enthalpy controlled.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2002年第7期1279-1283,共5页
Acta Chimica Sinica
基金
国家自然科学基金 (Nos .2 9976 0 0 4
2 0 136 0 2 0 )
教育部优秀青年教师基金 (No .710 6 7)资助项目
