摘要
目的 探讨湖南地区 HL A- B2 7对强直性脊柱炎的诊断意义。 方法 对临床疑为强直性脊柱炎的患者静脉血采用玻璃珠抗凝分离淋巴细胞并用标准的 Terasaki软滴法作微量淋巴细胞毒试验。结果疑为强直性脊柱炎患者 6 47例 ,其中男性 481例 ,女性 16 6例 ,男女比例为 3:1。 B2 7阳性率为 5 6 .5 7% ,其中男性占 6 7.15 % ,女性占 2 5 .90 %。发病年龄主要集中在青少年时期。 结论 HL A- B2 7血清学检测对强直性脊柱炎的诊断和鉴别诊断具有重要意义。
Objective To evaluate the diagnostic significance of HLA-B27 antigen detection in patients with skeptical ankylosing spondylitis(AS) in Hunan province. Methods Peripheral blood mononuclear cells(PBMC) were isolated from venous blood of the patients with skeptical AS by Ficoll-Hypaque density centrifugation, and the blood samples were anticoagulated by glass beads. The HLA-B27 typing was performed by micro-lymphocyte cytotoxic test with standard soft drop assay. Result Among 647 patients with skeptical AS,male and femal were481 and 166 respectively.The male to femal ratio was about 3:1,and the percentage of HLA-B27 position was 67.15% and 25.90% respectively.Clinical manifestation of ankylosing spondylitis usually begin in late adolescence. Conclusion HLA-B27 antigen detection is an effective method on AS diagnosis and differential diagnosis.
出处
《实用预防医学》
CAS
2002年第3期218-219,共2页
Practical Preventive Medicine
