摘要
目的 通过试验菌株与对照菌株相比较 ,观察其生物学性状 ,确定试验菌株的生态型。方法 选择生物化学试验、毒力决定因子、营养型、毒力、质粒及外膜蛋白等项目。结果 试验菌株与对照菌株生物学性状有较大差异 ,生化性状为鼠李糖 +、甘油 +、脱氮 -、麦芽糖 +、阿胶糖 -、密二糖 +,毒力因子是鼠疫菌荚膜抗原(Fra I+ )、毒力抗原 (Vwa+ )、鼠疫菌素 I(Pst I+ )、色素沉着因子 (Pgm+ ) ,营养型为精氨酸、亮氨酸依赖型 (Arg- 、L eu- ) ,质粒为 6、4 5、6 5 Mdal质粒等。结论 确定了我国一个新的鼠疫菌生态型
Objective To determine the ecotype of the tested strains of yersinia pestis (Y.pestis) by comparing and observing the tested and control strains biological characters.Methods The tests of biochemical , virulence determinants, nutrient type, virulence, plasmids and outer membrane protein were conducted.Results The distinctive difference existed on biological character between the tested strains and control one.In the strains from Shiqu Sichuan the biochemical characters are rhamnose+, maltose+, melibinose+, arabinose+ and nitrate reduction-. The virulent determinants are FractionI +, Vw antigen +, PasticinI +, and Pigmentation +. The nutrient types are Arginine - and Leucine -.There are three kinds of plasmids, 6 Mdal, 45 Mdal and 65 Mdal.Conclusions A new ecotype of Y.pestis was determined.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2002年第4期269-272,共4页
Chinese Jouranl of Endemiology
基金
卫生部疾病控制司基金资助项目 (2 0 0 0 -0 1-0 1)
