摘要
目的 :探讨地多糖的提取分离 ,以便研究其药理功效。方法 :采用Sevage法、DEAE -纤维素 (DEAE -5 2 )离子交换层析法及SephadexG - 10 0凝胶过滤法提纯地多糖 (MD1)。纯化的地多糖MD1的结构经TLC、UV、IR、GC、HPLC方法以及高碘酸氧化、Smith降解等方法进行测定。结果 :地多糖MD1是有许多支链的中性多糖 ,其组成为Rha∶Xyl∶Ara∶Man∶Glc∶Gal =1∶ 1.7∶ 1.9∶ 2 4 .5∶ 12 .3∶ 3.5 (摩尔比 ) ,分子量为 3.1× 10 3 。
Objective:Polysaccharide MD 1 was isolated and purified by decocting with water,followed by Sevage method,alcohol precipitation,DEAE-cellulose ion exchange chromatography gel filtration chromatography(Sephadex G-100).The structure of the polysaccharide purified was determined by the methods of TLC,UV,IR,GC,HPLC,periodate oxidation,Smith degration,etc. MD 1 is a kind of neutral heteropolysaccharide with many branches.It consists of Rha,Xyl,Ara,Man,Glc and Gal in a molar rate of 1∶1.7∶1.9∶24.5∶12.3∶3.5. The average MW was 3.1×10 3.
出处
《广州医学院学报》
2002年第2期20-23,共4页
Academic Journal of Guangzhou Medical College
关键词
地nie
地nie多糖
纯化
分析
中药
Melastoma dodecandrum Lour
polysaccharide
purification
analysis
