摘要
目的：从枸杞子中分离枸杞多糖，并研究其组成性质。方法：采用ＤＥＡＥ纤维素柱层析和凝胶柱层析法分离纯化枸杞多糖，采用气相色谱法、比色法、氨基酸自动分析、元素分析、红外光谱分析等方法对其组成进行研究。结果：分离得到ＬＢＰ－Ⅱ、ＬＢＰ－Ⅲ、ＬＢＰ－Ⅳ３种水溶性多糖均由鼠李糖、半乳糖、葡萄糖、甘露糖、木糖、半乳糖醛酸与１７种氨基酸组成，总糖含量分别为８２．９９％，８３．９９％，８４．７６％，氨基酸总量分别为１１．５１％，８．４６％，８．２７％，３种多糖分子量均大于２００００。结论：ＬＢＰ－Ⅱ、ＬＢＰ－Ⅲ、ＬＢＰ－Ⅳ都是醚性杂多糖同多肽或蛋白质构成的复合多糖。
OBJECTIVE:To study the isolation and composition of Lycium barbarum polysaccharides(LBP)METHODS:LBP was isolated from Lyciun1 barbarun1 by DEAE ion-exchange cellulose and Sephacryl gel chro-matography,and their structural composition was studied by means of GC,colormetry,amino acid automatic analy-sis, elemental analysis,IR,etc.RESULTS:Three water soluble polysaccharides of LBP-Ⅱ,LBP-Ⅲ and LBP-Ⅳwere obtained. They were composed of 6 kinds of monosaccharides,galacturonic acid and l 7 kinds of amino acids。The monosaccharides in LBP-Ⅱ,LBP-Ⅲ and LBP-Ⅳ contained rhamnose, galactose, glucose,arabinose,mannoseand xylose in mole ratio of 3. 57:l. 53 : l. 03 :1.00:0.22 :0.02,4.22:1.38:1.00 : 2. 43:0. 38:0. 95,3. 57:1.00 : 0.86 : 2.53: 0.29 : 1.33,respectively。The contents of carbohydrate were 82. 99%,83. 99%and 84. 67%respectively.The total contents of amino acids were ll. 5l%,8. 46%and 8. 27%respectively in the order of LBP-Ⅱ, LBP-Ⅲ and LBP-Ⅳ,The resuIts also showed that their molecular weights were all higher than 20 000.CON-CLUSION : Three LBP are complex polysaccharides consisting of acidic heteropolysaccharides and polypeptide orprotein。
出处
《中国药学杂志》
CAS
CSCD
北大核心
1996年第12期716-720,共5页
Chinese Pharmaceutical Journal
关键词
枸杞多糖
分离
纯化
组成
Lycium barbarum,Lycium barbarum polysaccharide,isolation,composition
