摘要
目的 研究异丙酚对PC12细胞膜流动性及 [Ca2 + ]i的影响。方法 以PC12细胞作为神经细胞模型 ,加入不同浓度的异丙酚 ,用荧光偏振法测定细胞膜微粘度 η的动态变化 ,用激光扫描共聚焦显微镜检测 [Ca2 + ]i 随时间变化曲线。结果 ①异丙酚各剂量组的 η值均降低 ,并呈现剂量依赖性 ;② 30、10 0mg·L-1的异丙酚在加药后 2 0～ 30s使[Ca2 + ]i 短暂升高 ,[Ca2 + ]i的峰值分别比加药前增加了119%和 14 0 % ,但在 5 0s内均恢复到加药前水平。
AIM To study the effects of propofol on membrane fluidity and intracellular free Ca 2+ concentration ([Ca 2+ ] i ) in PC12 cells and discuss its relevant mechanism. METHODS PC12 cell lines were divided into seven groups: control, solvent and propofols(1,3,10,30,100 mg·L -1 ). Fluorescence depolarization method was used to measure dynamically microviscosity in PC12 cells and [Ca 2+ ] i was detected using calcium fluorescentprobe Fluo 3/AM and a laser scanning confocal microscope. RESULTS ①Acute administration of various doses of propofol induced a significant decrease of microviscosity in PC12 cells dose dependenty. ② Solvent, propofol at dose of 10 mg·L -1 had no effect on [Ca 2+ ] i in PC12 cells, however, after 30 and 100 mg·L -1 administration, [Ca 2+ ] i increased markedly at 20～30 seconds (increase percentage were 119% and 140% respectively) and then recovered to their pre administration levels within 50 seconds. CONCLUSION The propofol can significantly increase membrane fluidity in PC12 cells in a dose dependent manner and elevate [Ca 2+ ] i in PC12 cells at doses of 30 and 100 mg·L -1 . These changes are consistent with each other and related closely with anesthetic effect of propofol.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2002年第3期264-266,共3页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助课题 No 3 9970 715
江苏省自然科学基金资助课题NoBK2 0 0 114 3
