摘要
在大鼠饲料中加入硒酸钠(Na_2SeO_4),使其硒含量分别为0.2,2.0和5.0ppm,每一剂量水平又分为补充与不补充维生素E(VE)和蛋氨酸(Met)组。7周后,观察VE和Met对不同硒水平大鼠代谢的影响,并探讨可能存在的解毒机制。结果显示:未补充VE-Met的5.0ppm高硒组大鼠出现了硒中毒,补充组有明显的解毒效果,血、肝、肾和骨骼肌等组织硒含量显著下降,生化指标好转,尿三甲基硒离子硒(TMSe^+——Se)排出率由不补充组的22％上升为33％(P<0.05),但对其它补充组无此作用。表明:VE-Met联合使用对硒中毒有解毒作用,其机制是加强了机体硒的甲基化去毒能力,推测,除尿TMSe^+外,呼气中的二甲基硒(DMSe)也可能有所增加。
The weaning male Wistar rats were fed diets with 0.2, 2.0 and 5.0 ppm as Na2SeO4 respectively for 7 weeks. On each Se level, rats were divided into groups with or without supplementation of vitamin E and DL-methi-onine (VE-Met) and possible metabolic interactions of VE-Met to dietary Se at different levels in relation to the detoxification mechanism were ob- served. The results showed that only rats in unsupplemented, 5 ppm Se-diet group exhibited distinct toxic manifestations, and supplementation of VE-Met to this group gave effective protection against the toxic action. In the supplemented group Se content of blood, liver, kidneys and skeletal muscle of the experimental animals was decreased, while the excretory rate of urinary trimethylselenonium ion was increased to a level of 33% in contrast to 22% in the unsupplemented group (P<0.05). For the rest supp-lemented groups (0.2 and 2.0 ppm), however, amounts of trimethylselenonium excreted in urine were not more than those in their respective control groups. It thus demonstrated that VE-Met would play a role in Se detoxification via the mechanism of promoting methylation of Se metabolite. To account for the total excretion of Se, it is suggested that methylated products, such as dimethylselenide may be also increased in respiration as well, during detoxification.
出处
《营养学报》
CAS
CSCD
北大核心
1991年第1期32-38,共7页
Acta Nutrimenta Sinica
关键词
蛋氨酸
维生素E
解毒机制
硒
Se detoxification vitamin E DL-methionine trimethylselenonium ion
