摘要
本试验用昆明系种小鼠作为试验动物进行60日饲养试验以评价粒度为100nm纳米硒的亚慢性毒性。144只体重17～18g昆明系小鼠,分成4组,每组6个重复(3个重复为公鼠,3个重复为母鼠),每个重复6只鼠。第1组至3组分别在基础日粮中添加纳米硒260、130、65mg/kg体重,第4组为对照组饲喂基础日粮。260、130、65mg/kg体重各添加量均表现出生长抑制作用,高剂量(260mg/kg体重)纳米硒组的小鼠毒性发作早,试验Ⅰ期(30d)累计死亡率高达16.7%,GSH-Px活性显著低于65mg/kg体重纳米硒添加组(P<0.05)。低剂量(65mg/kg体重)纳米硒则表现出慢性毒性,试验Ⅱ期(60d)小鼠血浆中GSH-Px活力下降,4周小鼠累计死亡率为55.6%。
Sub-chronic toxicity test was conducted to assess potential toxicity of Nano-Se particles in sizes of 100 nm. A total of 144 Kunming mice （body weight of 16--17 g） were randomly divided into 4 treatment groups. Each group consisted of 6 replicates （3 male and 3 female） with 6 mice per replicate. Supplement of Nano-Se in 3 treatment groups was 260, 130, and 65 mg/kg, respectively. Mice in group 4 were fed basal diet. Nano-Se caused growth inhibition. Onset of toxicity in group 1 （260 mg/kg supple- ment） was earlier, and the mortality was up to 16.7% during period I. Plasma GSH-Px activity of mice in group 1 was significantly lower （P〈0.05） than that of group3 （65 mg/kg supplement）. During period II, low Nano-Se supplement （65 mg/kg） had chronic toxicity. GSH-Px activity in 65 mg/kg supplement treat- ment decreased, and the mortality was 55.6~.
出处
《中国兽医杂志》
CAS
北大核心
2012年第10期19-21,共3页
Chinese Journal of Veterinary Medicine
基金
青岛市科技计划项目(08-1-3-14JCH)
