摘要
目的 探讨国产降纤酶对大鼠局灶性脑缺血 /再灌注动物模型的神经行为学和脑梗死体积的影响 ,以证明其是否有脑保护作用。方法 采用线栓法制作大鼠大脑中动脉闭塞模型 ,用 Zea L onga 5分制评分和 TTC染色法评价神经行为学和脑梗死体积。由吉林大学第一医院和复旦大学华山医院共同进行实验研究 ,每部分实验动物各做一半。动物随机分为缺血 3h及再灌注 3h、6 h、2 4h和 72 h;缺血 6 h及再灌注 3h、6 h、2 4h;缺血 2 4h等共 10组。降纤酶采用 8U / kg腹腔注射给药。结果 行为学结果 :在 10组中仅于缺血 6 h再灌注 6 h组 Zea L onga评分有明显改善 (P<0 .0 5 ) ,其余各组与盐水对照组比较无显著性差异。梗死体积测定结果 :在缺血 3h各组中 ,只有缺血3h再灌注 72 h这个时间点比盐水组无明显缩小 (P>0 .0 5 ) ,其余各时间点梗死体积均明显缩小 (P分别 <0 .0 5、0 .0 1和 0 .0 0 1)。在缺血 6 h和 2 4h组中 ,各时间点与盐水组比较均明显缩小 (分别为 P<0 .0 1和 P<0 .0 5 )。结论 国产降纤酶对大鼠局灶性脑缺血及再灌注损伤有一定的保护作用。
Objective To explore influence of homemade defibrase on cerebral infarct volume and behavior after focal ischemia and reperfusion in rats and to confirm its cerebral protection. Methods Animal model of focal cerebral ischemia and reperfusion were made by thread. Zea Longa method was used to evaluate behavioral change and TTC staining was applied to show cerebral infarction. Results Zea Longa evaluation showed no difference between the groups treated with homemade defibrase and the control groups except in the group of 6h reperfusion following 6h cerebral ischemia. In that group,Zea Longa scores in treated animals were lower than those in control(P<0.05). Cerebral infarct volume in treated groups that underwent ischemia for 3h,6h and 24h were decreased(P<0.05,P<0.01 and P<0.001,respectively) except the group underwent ischemia for 3h and reperfusion for 72h. Conclusion homemade defibrase may have neuroprotective effect on cerebral ischemic damage induced by focal cerebral ischemia and reperfusion in rats.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2002年第3期134-136,共3页
Journal of Apoplexy and Nervous Diseases
基金
卫生部科研基金课题 ( 98-2 -390 )
