摘要
目的 比较脑内移植脑源性神经营养因子 (BDNF)载体细胞对新生大鼠缺氧缺血性脑损伤后脑内不同部位的作用。 方法 7日龄新生大鼠随机分为假手术组 (C)、缺氧缺血 +对照细胞移植组 (A)和缺氧缺血 +载体细胞移植组 (B) ,在脑缺氧缺血性损伤后即刻脑内植入构建的 BDNF载体细胞 ,2 1d后比较不同部位脑萎缩程度并进行组织学评分。 结果 损伤后 2 1d,A、B组伤侧不同部位皮层、海马和纹状体均明显萎缩 ,体积比 C组或同组对侧缩小 ,但与 A组比较 ,B组移植位置附近皮层和左侧其它部位脑组织的萎缩程度均显著减轻。组织学评分也有类似的结果。 结论 脑内移植 BDNF载体细胞对损伤脑组织有较好的保护作用 。
Objective To investigate the neuroprotection of different brain regions after transplantation of genetically modified myoblasts producing brain derived neurotrophic factor (BDNF) into cortices of neonatal rats subjected to hypoxic ischemic encephalopathy (HIE). Methods Seven day old pups were randomized into sham operated group (C), HIE+BDNF group (B) and HIE+mock transplantation group (A). A rat myoblast cell line expressing and secreting BDNF (BDNF(+)/L 6TG) was constructed. A stereotaxical intracerebroparenchymal transplantation of either BDNF(+)/L 6TG (B) or BDNF(-)/L 6TG (absence of BDNF, A) at 0.8 μl of cell suspension (4×10 4/μl) into the left cortex of the brain was carried out shortly after HIE undergone by ligation of left common carotid artery followed by a 2.5 h inhalation of humidified 8% O 2+92% N 2 at 37℃. Changes of areas of different brain regions of cortex, hippocampus and striatum at different sections were observed 21 d after the procedure. Injury severity scores of brain tissues were also performed. Results Twenty one days after the manipulation, various degrees of atrophy were observed in the three regions of left hemispheres in group A or B versus contralateral corresponding regions or group C whereas this atrophy in group B was significantly reduced when compared to group A. Not only was the atrophy of cortex near the grafting point alleviated but some other regions of left hemispheres. A similar change pattern was seen in injury severity scores of the tissue. Conclusion Present data suggest that intracerebral transplantation of genetically modified myoblasts producing BDNF has a beneficial effect on the protection from damage of both circumscriptus brain tissues and some other areas distanced from the grafted place.
出处
《中华围产医学杂志》
CAS
2002年第2期143-146,共4页
Chinese Journal of Perinatal Medicine
基金
全军"九.五"医学科研规划科研基金资助项目 (基金编号 98D0 2 2 )
关键词
脑内移植
BDNF载体细胞
新生大鼠
缺氧缺血性脑损伤
影响
Brain derived neurotrophic factor
Cerebral anoxia
Cerebral ischemia
Rats,sprague dawley
Cell transplantation
