摘要
目的：探讨强啡肽Ａ１～１３在新生鼠脑缺氧、缺血性损伤中的作用。方法：将７日龄新生鼠右侧颈总动脉结扎并在低氧环境下制成半球性脑缺氧、缺血模型，观察脑损伤后不同时间、不同脑区强啡肽Ａ１～１３免疫活性物质含量的改变，和小脑延髓池注射强啡肽Ａ１～１３抗血清对脑损伤病理过程的影响。结果：新生鼠脑组织缺氧、缺血后不同时间，强啡肽Ａ１～１３含量在皮层、下丘脑和海马回均显著升高，以大脑皮层含量升高最为明显；小脑延髓池注射８μｌ强啡肽Ａ１～１３抗血清，可明显减轻损伤后脑水肿，改善新生鼠的一般状况。结论：强啡肽Ａ１～１３参与脑缺氧、缺血的病理过程，过量的强啡肽Ａ１～１３有加重脑缺氧、缺血后病理改变的作用。
Objectives: To study the secondary pathological changes in the central nervous system after injury and the role of dynorphin A 1￣13 in hypoxi ischemic brain injury in neonatal rats. Methods: Changes of concentrations of dynorphin A 1￣13 immunoreactives in some brain areas at different times after brain injury and the effect of injecting 8 μl dynorphin A 1￣13 anti serum into the medulla pool on pathological process of brain injury were observed in perinatal cerebral hypoxia ischemic rat models prepared by permanent ligation of right common carotid artery combined with a temporary systematic hypoxia at 37℃ in 7 day old Wistar rats. Results: Concentrations of dynorphin A 1￣13 in cortex, hypothalamus and hippocampus increased significantly after the injury. Microinjection of anti dynorphin A 1￣13 serum into the medulla pool 1h preinjury might markedly reduce brain edema and improve the physiological condition. Conclusions: Dynorphin A 1￣13 plays a role in the pathophysiological process of brain hypoxia ischemia and excessive amount of dynorphin A 1￣13 had some detrimental effect on the process.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1997年第4期198-201,共4页
Chinese Journal of Obstetrics and Gynecology
